Myozenin 3 CRISPR/Cas9 KO Plasmid (h): sc-406184
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myozenin 3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myozenin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myozenin 3 CRISPR/Cas9 KO Plasmid (h) | sc-406184 | 20 µg | $397.00 |
Overview
MYOZ3 encodes myozenin 3, a Z-disc–associated protein that contributes to sarcomeric organization by linking structural components with signaling complexes in striated muscle. Myozenin family members interact with α-actinin and modulators of calcineurin–NFAT signaling, implicating MYOZ3 in pathways that couple mechanical load to transcriptional remodeling and muscle fiber maintenance. Dysregulation of Z-disc architecture and calcineurin-dependent signaling is relevant to studies of myofibrillar integrity, contractility, and stress-adaptive responses in skeletal and cardiac muscle. MYOZ3 therefore serves as a useful target for dissecting cytoskeletal–signal transduction crosstalk in muscle biology and related disease-model systems.
Myozenin 3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYOZ3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYOZ3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYOZ3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myozenin 3 protein expression.
This CRISPR knockout system enables efficient generation of MYOZ3-deficient cell models for investigation of Myozenin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYOZ3 exon(s) critical for Myozenin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYOZ3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myozenin 3 CRISPR/Cas9 KO Plasmid (h) and Myozenin 3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYOZ3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myozenin 3 HDR Plasmid (h) and Myozenin 3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYOZ3 homology arms to support homology-directed repair at defined MYOZ3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.