Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myosin Ic Double Nickase Plasmid (h): sc-403013-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin Ic Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Myosin Ic Double Nickase Plasmid (h) and Myosin Ic Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MYO1C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myosin Ic Antibody (13): sc-136544
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin Ic Double Nickase Plasmid (h) | sc-403013-NIC | 20 µg | $410.00 | |||
Myosin Ic Double Nickase Plasmid (h2) | sc-403013-NIC-2 | 20 µg | $410.00 |
Human MYO1C encodes myosin Ic, an actin-based motor that links the cortical cytoskeleton to membranes to support membrane trafficking, cell shape changes, and mechanotransduction. Myosin Ic contributes to endocytic and exocytic transport, cortical tension regulation, and actin-dependent remodeling at the plasma membrane, integrating signals that influence adhesion and motility. Through these functions, MYO1C is commonly studied in pathways governing vesicle dynamics, cytoskeletal organization, and receptor trafficking. Dysregulation of these processes has been associated with altered cell migration and signaling behaviors relevant to cancer biology, metabolic regulation, and neurobiology research.
Myosin Ic Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MYO1C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MYO1C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MYO1C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MYO1C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.