Myosin Ia CRISPR/Cas9 KO Plasmid (m): sc-436599
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin Ia CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myosin Ia genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin Ia CRISPR/Cas9 KO Plasmid (m) | sc-436599 | 20 µg | $397.00 |
Overview
Myo1a encodes myosin Ia, an actin-based motor enriched in polarized epithelial cells where it supports microvillar structure and membrane–cytoskeleton coupling at the apical surface. Myosin Ia contributes to actin filament dynamics, membrane tension, and vesicle trafficking processes that help maintain brush border organization and epithelial barrier function. Through interactions with the cortical actin network, it influences pathways governing cell polarity, absorption, and mechanical responses in intestinal epithelium. Altered MYO1A function or expression has been associated with epithelial dysregulation and has been investigated in gastrointestinal pathobiology, including contexts of barrier impairment and tumorigenesis.
Myosin Ia CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Myo1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Myo1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Myo1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myosin Ia protein expression.
This CRISPR knockout system enables efficient generation of Myo1a-deficient cell models for investigation of Myosin Ia signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Myo1a exon(s) critical for Myosin Ia function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Myo1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myosin Ia CRISPR/Cas9 KO Plasmid (m) and Myosin Ia CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Myo1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myosin Ia HDR Plasmid (m) and Myosin Ia HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Myo1a homology arms to support homology-directed repair at defined Myo1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.