myogenin Antibody (5FD): sc-52903

myogenin Antibody (5FD) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 144-158 of rat myogenin, with reactivity in mouse, rat, and human species. anti-myogenin antibody (5FD) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Myogenin, also known as MYOG or MyoD1, is a member of the myogenic regulatory factor (MRF) family, including MyoD, Myf-5, and Myf-6 (MRF4 or herculin), and is essential for skeletal muscle differentiation. Myogenin is predominantly localized to the nucleus, where myogenin functions as a basic helix-loop-helix (bHLH) transcription factor that regulates the expression of genes required for muscle-specific terminal differentiation. Nuclear localization of myogenin is critical for binding to E-box consensus sequences (CANNTG) in the promoter regions of target genes, ensuring precise transcriptional activation during myogenesis. Myogenin expression is upregulated as myoblasts transition to differentiated muscle cells, playing a central role in muscle fiber formation and regeneration. Alterations in myogenin expression or localization can impair muscle development and repair, highlighting myogenin′s importance in maintaining functional skeletal muscle. myogenin (5FD) monoclonal antibody serves as a valuable tool for studying the cellular mechanisms of muscle development and the regulation of gene expression in myogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-myogenin Antibody (5FD) has 82 citations in a variety of scientific publications.

Primary antibodies like Anti-myogenin Antibody (5FD) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

myogenin Antibody (5FD) Specifications

Alternate Product Name: myogenin

Product Clone Name: 5FD

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 144-158

Epitope Target: myogenin

Epitope Species: rat

Product Reactivity/Detection: myogenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 82 citations in various scientific publications

Available Conjugates/Forms for myogenin Antibody (5FD): AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred myogenin antibody is myogenin Antibody (F5D): sc-12732.