MYLK2 Antibody (MY-21): sc-58803

MYLK2 Antibody (MY-21) is a mouse monoclonal IgM antibody that detects MYLK2 protein of human, avian, bovine, and porcine origin by western blotting (WB) and immunofluorescence (IF). Anti-MYLK2 antibody (MY-21) is available as a non-conjugated format, providing researchers with a versatile tool for studying this important protein. Myosin light chain kinase 2 (MYLK2) plays a crucial role in muscle contraction by phosphorylating myosin light chains, which is essential for the regulation of smooth and cardiac muscle activity. The phosphorylation of myosin light chains is a key step in the excitation-contraction coupling process, where an increase in intracellular calcium levels leads to muscle contraction. Understanding MYLK2 function is vital, as dysregulation can contribute to various cardiovascular diseases and muscle disorders. MYLK2 is also involved in signaling pathways that affect cell motility and proliferation, making MYLK2 a significant target for research in both muscle physiology and pathology. The presence of multiple isoforms of myosin light chains, each associated with different muscle types, highlights the complexity of muscle regulation and the importance of MYLK2 in maintaining muscle function.

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MYLK2 Antibody (MY-21) References:

1. Abnormal cardiac structure and function in mice expressing nonphosphorylatable cardiac regulatory myosin light chain 2.  |  Sanbe, A., et al. 1999. J Biol Chem. 274: 21085-94. PMID: 10409661
2. The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation.  |  Davis, JS., et al. 2001. Cell. 107: 631-41. PMID: 11733062
3. Rejection of pancreatic islet xenografts does not require CD8+ T-lymphocytes.  |  Gill, RG. and Coulombe, M. 1992. Transplant Proc. 24: 2877-8. PMID: 1465980
4. Myosin light chain 1 isoforms in slow fibers from global and orbital layers of canine rectus muscles.  |  Bicer, S. and Reiser, PJ. 2004. Invest Ophthalmol Vis Sci. 45: 138-43. PMID: 14691165
5. Perineal abscess after prostatic urethroplasty with balloon catheter: report of a case.  |  Castaneda, F., et al. 1990. Radiology. 174: 49-50. PMID: 1688468
6. Development of an immunoradiometric assay kit for ventricular myosin light chain I with monoclonal antibodies.  |  Katoh, H., et al. 1992. Clin Chem. 38: 170-1. PMID: 1733600
7. Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q 32.1-2qter.  |  Cohen-Haguenauer, O., et al. 1988. Hum Genet. 78: 65-70. PMID: 3422212
8. The complete nucleotide sequences of cDNA clones coding for human myosin light chains 1 and 3.  |  Seidel, U., et al. 1987. Nucleic Acids Res. 15: 4989. PMID: 3601661
9. The myosin alkali light chain proteins and their genes.  |  Barton, PJ. and Buckingham, ME. 1985. Biochem J. 231: 249-61. PMID: 3904738