Date published: 2025-11-1

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MYL3 Antibody (MLM544): sc-58805

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Datasheets
  • MYL3 Antibody (MLM544) is a mouse monoclonal IgG2b provided at 100 µg/ml
  • raised against myosin light chain 3 of human origin
  • recommended for detection of ventricular myosin light chain 3 of mouse, rat, human, bovine and porcine origin by WB and IP
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MYL3 Antibody (MLM544). This work is in progress.

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    MYL3 Antibody (MLM544) is a mouse monoclonal IgG2b antibody that detects myosin light chain 3 in mouse, rat, human, bovine, and porcine samples through applications such as western blotting (WB) and immunoprecipitation (IP). Anti-MYL3 antibody (MLM544) plays a crucial role in understanding the function of myosin light chain 3, which is essential for muscle contraction and specifically involved in the regulation of cardiac and skeletal muscle function. Myosin light chain 3, encoded by the MYL3 gene, is a key component of the myosin complex, which is vital for muscle contraction as myosin light chain 3 interacts with myosin heavy chains to facilitate the sliding filament mechanism. The phosphorylation state of myosin light chain 3 is critical, as phosphorylation influences the contractile properties of muscle fibers; thus, understanding this regulation can provide insights into various cardiac and skeletal muscle disorders. Additionally, myosin light chain 3 is associated with different muscle types, and expression can serve as a cardiac marker, making myosin light chain 3 significant in both physiological and pathological contexts. MYL3 (MLM544) monoclonal antibody′s ability to effectively detect myosin light chain 3 across multiple species enhances research utility in comparative studies of muscle biology and disease.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    MYL3 Antibody (MLM544)

    sc-58805
    100 µg/ml
    $316.00

    I am using MYL3 (MLM544): sc-58805 antibody for immunoprecipitation, how much antibody should I use?

    Asked by: Cweed
    Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Positive WB data in rat skeletal musclePositive WB data in rat skeletal muscle, mouse heart and rat heart tissue extracts. -SCBT QC
    Date published: 2014-09-01
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    MYL3 Antibody (MLM544) is rated 4.0 out of 5 by 1.
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