MYH1/2 Antibody (A4.1025): sc-53088

MYH1/2 Antibody (A4.1025) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect myosin heavy chain proteins encoded by the MYH1 and MYH2 genes, also known as MyHC-IIx and MyHC-IIa, respectively. These proteins are localized within the sarcomeric thick filaments of skeletal muscle cells, where they play a pivotal role in muscle contraction by interacting with actin to generate force and facilitate movement. The precise cellular localization of MYH1 and MYH2 is crucial for determining the contraction speed and functional properties of different muscle fiber types, making anti-MYH1/2 antibody (A4.1025) an essential tool for muscle physiology studies. Capable of recognizing MYH1/2 across a diverse range of species including mammals, avians, amphibians, and fish, MYH1/2 monoclonal antibody (A4.1025) is recommended for use in western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Available in both non-conjugated form and various conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, MYH1/2 (A4.1025) antibody offers versatility for a wide array of experimental setups. By enabling precise detection and localization of MYH1 and MYH2 isoforms, anti-MYH1/2 antibody (A4.1025) supports advanced research into muscle development, muscle disease mechanisms, and the molecular dynamics of muscle function.

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MYH1/2 Antibody (A4.1025) References:

1. Muscle fiber pattern is independent of cell lineage in postnatal rodent development.  |  Hughes, SM. and Blau, HM. 1992. Cell. 68: 659-71. PMID: 1531450
2. Identification of three developmentally controlled isoforms of human myosin heavy chains.  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
3. Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA.  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
4. Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis.  |  Nagai, R., et al. 1989. J Biol Chem. 264: 9734-7. PMID: 2722872
5. Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining.  |  Wang, C., et al. 2017. Bio Protoc. 7: PMID: 28752107
6. A missense mutation in MYH1 is associated with susceptibility to immune-mediated myositis in Quarter Horses.  |  Finno, CJ., et al. 2018. Skelet Muscle. 8: 7. PMID: 29510741
7. Characterization of a human perinatal myosin heavy-chain transcript.  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
8. Regulation of differentiation of vascular smooth muscle cells.  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
9. Phylogenetic analysis of the myosin superfamily.  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
10. Mechanisms of smooth muscle contraction.  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
11. The mammalian myosin heavy chain gene family.  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MYH1/2 Antibody (A4.1025) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-MYH1/2 Antibody (A4.1025) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MYH1/2 Antibody (A4.1025) Specifications

Alternate Product Name: MYH1/2

Product Clone Name: A4.1025

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: adult skeletal muscle myosin

Epitope Species: human

Product Reactivity/Detection: myosin heavy chains encoded by MYH1 and 2

Species Reactivity/Detection: mammal, avian, amphibian, fish

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for MYH1/2 Antibody (A4.1025): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred antibody is Antibody (): sc-32732m.