Date published: 2026-4-24

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MYH1/2/3 Antibody (N3.36): sc-53092

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Datasheets
  • MYH1/2/3 Antibody (N3.36) is a mouse monoclonal IgM κ MYH1/2/3 antibody, cited in 8 publications, provided at 200 µg/ml
  • raised against neonatal skeletal muscle myosin of human origin
  • recommended for detection of myosin heavy chains encoded by MYH1, 2 and 3 of mouse, rat, human, rabbit and fish origin by WB, IP, IF and IHC(P)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MYH1/2/3 Antibody (N3.36). This work is in progress.
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MYH1/2/3 Antibody (N3.36) is a mouse monoclonal IgM kappa light chain antibody engineered to detect myosin heavy chain proteins encoded by the MYH1, MYH2, and MYH3 genes, corresponding to MyHC-IIx, MyHC-IIa, and MyHC-embryonic isoforms, respectively. These myosin heavy chains are crucial components of the sarcomeric thick filaments within skeletal muscle cells, where their hexameric structure, comprising two heavy chains and four light chains, enables interaction with actin to generate the force necessary for muscle contraction. The intricate structure of MYH1, MYH2, and MYH3 is vital for determining the contraction speed and functional properties of different muscle fiber types, making anti-MYH1/2/3 antibody (N3.36) an essential tool for investigating muscle physiology and development. MYH1/2/3 (N3.36) antibody is reactive across multiple species, including mouse, rat, human, rabbit, and fish, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). By facilitating the precise detection and analysis of various myosin heavy chain isoforms, MYH1/2/3 monoclonal antibody (N3.36) supports advanced research into muscle development, muscle disease mechanisms, and the molecular dynamics that drive muscle function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MYH1/2/3 Antibody (N3.36)

sc-53092
200 µg/ml
$322.00

I am using MYH1/2/3 (N3.36): sc-53092 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_4, d_23, h_7CST
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Rated 5 out of 5 by from This clone was published for IHC in rat cardiacThis clone was published for IHC in rat cardiac fibroblasts. -SCBT Publication Review
Date published: 2015-04-13
Rated 5 out of 5 by from Excellent results using the antibody in formalinExcellent results using the antibody in formalin fixed, paraffin-embedded human skeletal muscle tissue showed cytoplasmic staining of myocytes. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Positive results by Western blot in mousePositive results by Western blot in mouse lung fibroblasts -SCBT Publication Review
Date published: 2015-01-18
Rated 4 out of 5 by from Specific signal by Western blot in rat skeletalSpecific signal by Western blot in rat skeletal muscle tissue extract. -SCBT QC
Date published: 2014-10-31
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MYH1/2/3 Antibody (N3.36) is rated 4.8 out of 5 by 4.
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