MYH3 Antibody (F1.652): sc-53091

MYH3 Antibody (F1.652) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the myosin heavy chain encoded by the MYH3 gene, also known as muscle embryonic myosin heavy chain or MYHCE, in mouse, rat, human, and rabbit samples. Myosin heavy chain 3 (MYH3) is a crucial component of the thick filaments in myofibrils, playing a vital role in muscle contraction and the development of skeletal muscle during the embryonic stage. MYH3 is primarily expressed in embryonic skeletal muscle, where MYH3 facilitates the formation of functional muscle fibers. As muscle fibers mature postnatally, MYH3 expression is gradually replaced by other myosin isoforms, such as MYH2, in mature skeletal muscle. Anti-MYH3 antibody (F1.652) targets an epitope of embryonic skeletal muscle myosin heavy chain, ensuring robust and accurate detection across various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Understanding the function and regulation of MYH3 is essential for studying muscle development, congenital muscle disorders such as distal arthrogryposis type 2B (DA2B), and muscle regeneration processes. MYH3 monoclonal antibody (F1.652) enables researchers to effectively investigate the expression patterns, localization, and interactions of MYH3, providing valuable insights into muscle physiology and pathology. Anti-MYH3 antibody (F1.652) is an indispensable tool for advancing research in muscle biology, developmental biology, and related biomedical fields, enabling the exploration of critical mechanisms that govern muscle formation and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MYH3 Antibody (F1.652) References:

1. Mutations in embryonic myosin heavy chain (MYH3) cause Freeman-Sheldon syndrome and Sheldon-Hall syndrome.  |  Toydemir, RM., et al. 2006. Nat Genet. 38: 561-5. PMID: 16642020
2. Identification of three developmentally controlled isoforms of human myosin heavy chains.  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
3. Smooth muscle myosin is composed of homodimeric heavy chains.  |  Kelley, CA., et al. 1992. J Biol Chem. 267: 2127-30. PMID: 1733920
4. Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA.  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
5. A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.  |  Cho, IC., et al. 2019. PLoS Genet. 15: e1008279. PMID: 31603892
6. Expanding the mutation and phenotype spectrum of MYH3-associated skeletal disorders.  |  Zhao, S., et al. 2022. NPJ Genom Med. 7: 11. PMID: 35169139
7. FOXO1 regulates bovine skeletal muscle cells differentiation by targeting MYH3.  |  Jiang, C., et al. 2024. Int J Biol Macromol. 260: 129643. PMID: 38253149
8. Characterization of a human perinatal myosin heavy-chain transcript.  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
9. Regulation of differentiation of vascular smooth muscle cells.  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
10. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.  |  Hughes, SM., et al. 1993. Dev Biol. 158: 183-99. PMID: 7687223
11. Phylogenetic analysis of the myosin superfamily.  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
12. The mammalian myosin heavy chain gene family.  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MYH3 Antibody (F1.652) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-MYH3 Antibody (F1.652) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MYH3 Antibody (F1.652) Specifications

Alternate Product Name: MYH3

Product Clone Name: F1.652

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MYH3

Epitope Species: human

Product Reactivity/Detection: myosin heavy chain encoded by MYH3

Species Reactivity/Detection: mouse, rat, human, rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for MYH3 Antibody (F1.652): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin