Myf-6 Antibody (G-7): sc-514379

Myf-6 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Myf-6 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Myf-6 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myf-6 protein, also known as MRF-4 or herculin, is a member of the myogenic regulatory factor (MRF) family, which plays a crucial role in muscle cell differentiation and development. Specifically, Myf-6 is involved in the regulation of muscle-specific gene expression, acting as a transcription factor that can form heterodimers with other bHLH proteins, such as Myo D and myogenin. This interaction is vital for the activation of muscle-specific genes during the differentiation process, ensuring proper muscle formation and function. Myf-6′s ability to bind to E box target sites in the regulatory regions of these genes highlights its importance in muscle development, making Myf-6 a key player in the intricate network of factors that govern myogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Myf-6 Antibody (G-7) References:

1. Identification and analysis of putative regulatory sequences for the MYF5/MYF6 locus in different vertebrate species.  |  Maak, S., et al. 2006. Gene. 379: 141-7. PMID: 16820272
2. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
3. Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.  |  Braun, T., et al. 1990. EMBO J. 9: 821-31. PMID: 2311584
4. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
5. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
6. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
7. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
8. MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice.  |  Braun, T., et al. 1994. Development. 120: 3083-92. PMID: 7720554
9. Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development.  |  Braun, T. and Arnold, HH. 1995. EMBO J. 14: 1176-86. PMID: 7720708
10. A cellular factor stimulates the DNA-binding activity of MyoD and E47.  |  Thayer, MJ. and Weintraub, H. 1993. Proc Natl Acad Sci U S A. 90: 6483-7. PMID: 8393567
11. Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination.  |  Hollenberg, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 8028-32. PMID: 8396258
12. Myf-5(m1)/Myf-6(m1) compound heterozygous mouse mutants down-regulate Myf-5 expression and exert rib defects: evidence for long-range cis effects on Myf-5 transcription.  |  Floss, T., et al. 1996. Dev Biol. 174: 140-7. PMID: 8626014

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myf-6 Antibody (G-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Myf-6 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myf-6 Antibody (G-7) Specifications

Alternate Product Name: Myf-6

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-242

Epitope Target: Myf-6

Epitope Species: human

Product Reactivity/Detection: Myf-6

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Myf-6 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent