Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mx1 Double Nickase Plasmid (h): sc-400977-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mx1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mx1 Double Nickase Plasmid (h) and Mx1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mx1 Antibody (E-5): sc-271024
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mx1 Double Nickase Plasmid (h) | sc-400977-NIC | 20 µg | $410.00 | |||
Mx1 Double Nickase Plasmid (h2) | sc-400977-NIC-2 | 20 µg | $410.00 |
MX1 encodes the interferon-induced dynamin-like GTPase Mx1, a key effector of innate antiviral immunity. Following type I and type III interferon signaling through the JAK–STAT pathway, Mx1 is upregulated as part of the interferon-stimulated gene program and can restrict replication of diverse RNA viruses by interfering with viral nucleocapsid trafficking and replication complexes. Mx1 activity links pathogen sensing to cellular stress and inflammatory transcriptional responses, and variation in interferon pathway tone involving MX1 has been investigated in the context of susceptibility to viral infection and interferon-associated immunopathology. As a canonical interferon response marker, MX1 is also used to monitor cytokine signaling dynamics and ISG network regulation in human cell models.
Mx1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.