MUTYH Antibody (C-6): sc-374571

MUTYH Antibody (C-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects MUTYH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MUTYH antibody (C-6) is available as the non-conjugated form. MUTYH, also known as mutY homolog (E. coli), plays a critical role as a DNA glycosylase mismatch repair enzyme, which is essential for maintaining genomic stability by correcting adenine residues that are mispaired with either oxidized (8-hydroxyguanines) or non-modified guanines. This process prevents mutations that can arise from oxidative DNA damage, a common issue in cellular environments exposed to reactive oxygen species. MUTYH (C-6) antibody helps detect MUTYH presence in neurons, where MUTYH helps protect mitochondrial genomes from oxidative stress throughout the lifespan of mammals. Multiple N-terminal splice variants of MUTYH in mammalian cells suggests a complex regulatory mechanism that may adapt MUTYH function in response to cellular conditions. Biallelic germ-line mutations in MUTYH are linked to an increased risk of colorectal adenomas and carcinomas, highlighting MUTYH significance in cancer biology. Increasing levels of MUTYH in A549 cells subjected to oxidative stress enhance cell survival, further emphasizing MUTYH protective role in cellular health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MUTYH Antibody (C-6) References:

1. Replication-associated repair of adenine:8-oxoguanine mispairs by MYH.  |  Hayashi, H., et al. 2002. Curr Biol. 12: 335-9. PMID: 11864576
2. Rat MYH, a glycosylase for repair of oxidatively damaged DNA, has brain-specific isoforms that localize to neuronal mitochondria.  |  Englander, EW., et al. 2002. J Neurochem. 83: 1471-80. PMID: 12472901
3. Germline mutations but not somatic changes at the MYH locus contribute to the pathogenesis of unselected colorectal cancers.  |  Halford, SE., et al. 2003. Am J Pathol. 162: 1545-8. PMID: 12707038
4. Developmental changes in expression and subcellular localization of the DNA repair glycosylase, MYH, in the rat brain.  |  Lee, HM., et al. 2004. J Neurochem. 88: 394-400. PMID: 14690527
5. A novel splice-site variant of the base excision repair gene MYH is associated with production of an aberrant mRNA transcript encoding a truncated MYH protein not localized in the nucleus.  |  Tao, H., et al. 2004. Carcinogenesis. 25: 1859-66. PMID: 15180946
6. Genetic alterations of the MYH gene in gastric cancer.  |  Kim, CJ., et al. 2004. Oncogene. 23: 6820-2. PMID: 15273732
7. N-terminus of the rat adenine glycosylase MYH affects excision rates and processing of MYH-generated abasic sites.  |  Ma, H., et al. 2004. Nucleic Acids Res. 32: 4332-9. PMID: 15310837
8. Myh deficiency enhances intestinal tumorigenesis in multiple intestinal neoplasia (ApcMin/+) mice.  |  Sieber, OM., et al. 2004. Cancer Res. 64: 8876-81. PMID: 15604247
9. Chromosomal instability in MYH- and APC-mutant adenomatous polyps.  |  Cardoso, J., et al. 2006. Cancer Res. 66: 2514-9. PMID: 16510566
10. Expanded extracolonic tumor spectrum in MUTYH-associated polyposis.  |  Vogt, S., et al. 2009. Gastroenterology. 137: 1976-85.e1-10. PMID: 19732775
11. MUTYH-associated colorectal cancer and adenomatous polyposis.  |  Yamaguchi, S., et al. 2014. Surg Today. 44: 593-600. PMID: 23605219
12. Management of familial adenomatous polyposis and MUTYH-associated polyposis; new insights.  |  Aelvoet, AS., et al. 2022. Best Pract Res Clin Gastroenterol. 58-59: 101793. PMID: 35988966

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MUTYH Antibody (C-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-MUTYH Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MUTYH Antibody (C-6) Specifications

Alternate Product Name: MUTYH

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 182-225

Epitope Target: MUTYH

Epitope Species: human

Product Reactivity/Detection: MUTYH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for MUTYH Antibody (C-6): N/A