Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Muskelin CRISPR/Cas9 KO Plasmid (h): sc-409182
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Muskelin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Muskelin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Muskelin Antibody (C-12): sc-398956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Muskelin CRISPR/Cas9 KO Plasmid (h) | sc-409182 | 20 µg | $397.00 |
Overview
MKLN1 encodes muskelin, a cytosolic and vesicle-associated scaffolding protein implicated in intracellular trafficking, cytoskeletal organization, and proteostasis. Muskelin participates in endosomal transport and cellular adhesion-related processes, and has been linked to regulation of receptor and ion channel turnover through coordination of multiprotein complexes. By influencing microtubule-dependent dynamics and subcellular localization of signaling components, MKLN1 can modulate pathways governing cell morphology, migration, and stress responses. Altered MKLN1/muskelin function has been studied in the context of neurobiology and cancer-associated phenotypes, supporting its relevance for mechanistic investigations of trafficking-dependent signaling and cellular homeostasis.
Muskelin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MKLN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MKLN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MKLN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Muskelin protein expression.
This CRISPR knockout system enables efficient generation of MKLN1-deficient cell models for investigation of Muskelin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MKLN1 exon(s) critical for Muskelin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MKLN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Muskelin CRISPR/Cas9 KO Plasmid (h) and Muskelin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MKLN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Muskelin HDR Plasmid (h) and Muskelin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MKLN1 homology arms to support homology-directed repair at defined MKLN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.