musculin Double Nickase Plasmid (h): sc-403242-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- musculin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- musculin Double Nickase Plasmid (h) and musculin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MSC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: musculin Antibody (4D7): sc-293482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
musculin Double Nickase Plasmid (h) | sc-403242-NIC | 20 µg | $410.00 | |||
musculin Double Nickase Plasmid (h2) | sc-403242-NIC-2 | 20 µg | $410.00 |
Human MSC encodes musculin, a basic helix–loop–helix (bHLH) transcription factor that regulates gene expression programs controlling lineage specification and cellular differentiation. Musculin modulates transcription through E-box–dependent DNA binding and interaction with other bHLH factors, linking it to developmental and cell fate pathways that influence proliferation and maturation states. Altered MSC expression has been reported across contexts involving dysregulated differentiation and transcriptional reprogramming, including cancer-associated phenotypes and immune or stromal cell state changes. These properties make musculin a useful target for mechanistic studies of transcriptional control, cell identity, and pathway cross-talk in human cell models.
musculin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MSC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MSC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MSC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MSC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.