musculin Antibody (4D7): sc-293482

Musculin Antibody (4D7) is a mouse monoclonal IgG2b kappa light chain antibody that detects musculin protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-musculin antibody (4D7) is available as the non-conjugated format. Musculin plays a crucial role in the regulation of myogenic differentiation, acting as a transcriptional repressor that modulates the activity of other myogenic factors such as Myo D. This regulation ensures proper timing of muscle-specific gene expression during myogenesis, allowing for the transition from undifferentiated myoblasts to fully differentiated muscle fibers. Musculin forms heterodimers with E family bHLH proteins, which are essential for binding to E box motifs in promoter regions of muscle-specific genes. Through this mechanism, musculin can inhibit transcriptional activation induced by Myo D, thereby delaying expression of muscle-specific genes until appropriate stages of differentiation. Expression of musculin remains notably high in undifferentiated and proliferating myoblasts, but decreases as myogenesis progresses, highlighting musculin′s importance in early stages of muscle development.

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musculin Antibody (4D7) References:

1. Evolutionary conservation of MyoD function and differential utilization of E proteins.  |  Zhang, JM., et al. 1999. Dev Biol. 208: 465-72. PMID: 10191059
2. Assignment of the human helix-loop-helix transcription factor gene musculin/activated B-cell factor-1 (MSC) to chromosome 8q21 and its mouse homologue (Msc) to the proximal region of chromosome 1.  |  Robb, L., et al. 1999. Genomics. 57: 318-9. PMID: 10198176
3. OUT, a novel basic helix-loop-helix transcription factor with an Id-like inhibitory activity.  |  Narumi, O., et al. 2000. J Biol Chem. 275: 3510-21. PMID: 10652346
4. Musculin/MyoR is expressed in kidney side population cells and can regulate their function.  |  Hishikawa, K., et al. 2005. J Cell Biol. 169: 921-8. PMID: 15967813
5. Musculin isoforms and repression of MyoD in muscle regeneration.  |  Zhao, P. and Hoffman, EP. 2006. Biochem Biophys Res Commun. 342: 835-42. PMID: 16500621
6. Transcriptomic changes and potential regulatory mechanism of intrauterine human chorionic gonadotropin co-cultured with peripheral blood mononuclear cells infusion in mice with embryonic implantation dysfunction.  |  Chen, J., et al. 2020. Ann Transl Med. 8: 99. PMID: 32175392
7. LncRNA Msc-AS1 facilitates lung adenocarcinoma through sponging miR-33b-5p to up-regulate GPAM.  |  Li, S., et al. 2021. Biochem Cell Biol. 99: 241-248. PMID: 33821667
8. Prioritization of non-coding elements involved in non-syndromic cleft lip with/without cleft palate through genome-wide analysis of de novo mutations.  |  Zieger, HK., et al. 2023. HGG Adv. 4: 100166. PMID: 36589413
9. Myf-5 and myoD genes are activated in distinct mesenchymal stem cells and determine different skeletal muscle cell lineages.  |  Braun, T. and Arnold, HH. 1996. EMBO J. 15: 310-18. PMID: 8617206
10. Muscle LIM protein promotes myogenesis by enhancing the activity of MyoD.  |  Kong, Y., et al. 1997. Mol Cell Biol. 17: 4750-60. PMID: 9234731
11. musculin: a murine basic helix-loop-helix transcription factor gene expressed in embryonic skeletal muscle.  |  Robb, L., et al. 1998. Mech Dev. 76: 197-201. PMID: 9767165
12. MyoR: a muscle-restricted basic helix-loop-helix transcription factor that antagonizes the actions of MyoD.  |  Lu, J., et al. 1999. Proc Natl Acad Sci U S A. 96: 552-7. PMID: 9892671

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-musculin Antibody (4D7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-musculin Antibody (4D7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

musculin Antibody (4D7) Specifications

Alternate Product Name: musculin

Product Clone Name: 4D7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-100

Epitope Target: musculin

Epitope Species: human

Product Reactivity/Detection: musculin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for musculin Antibody (4D7): N/A