Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MUS81 Double Nickase Plasmid (h): sc-402664-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MUS81 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MUS81 Double Nickase Plasmid (h) and MUS81 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUS81. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MUS81 Antibody (B-12): sc-376661
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MUS81 Double Nickase Plasmid (h) | sc-402664-NIC | 20 µg | $410.00 | |||
MUS81 Double Nickase Plasmid (h2) | sc-402664-NIC-2 | 20 µg | $410.00 |
MUS81 encodes a structure-specific endonuclease that forms a heterodimer with EME1/EME2 to resolve stalled replication forks and recombination intermediates such as Holliday junction-like structures. It functions within DNA damage response and replication stress pathways, coordinating with homologous recombination and checkpoint signaling to preserve genome stability. MUS81 activity contributes to chromosome segregation fidelity and limits accumulation of toxic DNA intermediates during S phase. Dysregulated MUS81-dependent processing has been linked to elevated genomic instability and altered sensitivity to replication stress in cancer-associated contexts, making it a common target in mechanistic studies of DNA repair.
MUS81 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUS81 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUS81. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUS81 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUS81-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.