MuRF1 (h): 293T Lysate: sc-369006

MuRF1 (h): 293T Lysate, derived from the HEK 293T cell line, is essential for researching the Muscle RING Finger-1 (MuRF1) protein, a key E3 ubiquitin ligase involved in muscle protein turnover. The 293T cell line, an enhanced version of HEK 293 cells, is genetically modified to express the SV40 large T antigen, facilitating high-efficiency replication and protein expression, making it ideal for studying proteins like MuRF1. Researchers utilize this lysate to investigate the role of MuRF1 in tagging specific muscle proteins for degradation via the ubiquitin-proteasome system, a process vital for maintaining muscle protein homeostasis. Studies often focus on understanding how MuRF1 targets sarcomeric proteins, such as myosin heavy chain, and other substrates for ubiquitination, influencing muscle protein degradation and turnover. Techniques such as immunoprecipitation, Western blotting, and mass spectrometry are employed to analyze the interactions and post-translational modifications mediated by MuRF1. By using MuRF1 (h): 293T Lysate, scientists can explore the regulatory mechanisms controlling muscle atrophy and hypertrophy, providing insights into the basic biological processes governing muscle maintenance and adaptation.