Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MULK Double Nickase Plasmid (m): sc-427577-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MULK Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MULK Double Nickase Plasmid (m) and MULK Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Agk. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MULK Antibody (F-3): sc-374390
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MULK Double Nickase Plasmid (m) | sc-427577-NIC | 20 µg | $410.00 | |||
MULK Double Nickase Plasmid (m2) | sc-427577-NIC-2 | 20 µg | $410.00 |
Mouse Agk encodes MULK, a mitochondrial acylglycerol kinase that phosphorylates monoacylglycerol and diacylglycerol to generate lysophosphatidic acid and phosphatidic acid, linking lipid metabolism to membrane biogenesis. MULK activity supports mitochondrial architecture, phospholipid remodeling, and bioenergetic homeostasis, with downstream effects on apoptotic susceptibility and cellular stress responses. Disruption of AGK/MULK function has been associated with impaired oxidative phosphorylation and altered lipid signaling, making the pathway relevant to studies of mitochondrial dysfunction and metabolic disease mechanisms. In mouse systems, Agk is also used to interrogate how mitochondrial lipid intermediates modulate organelle dynamics and signaling networks.
MULK Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Agk locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Agk. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Agk function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Agk-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.