mucolipin 1 CRISPR/Cas9 KO Plasmid (m): sc-430117
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mucolipin 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the mucolipin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mucolipin 1 CRISPR/Cas9 KO Plasmid (m) | sc-430117 | 20 µg | $397.00 |
Overview
Mcoln1 encodes mucolipin 1 (TRPML1), an endolysosomal Ca2+-permeable cation channel that regulates lysosome biogenesis, membrane trafficking, and autophagic flux. TRPML1-mediated Ca2+ release supports lysosome–autophagosome fusion, endocytic cargo processing, and signaling pathways linked to nutrient sensing and cellular stress responses. Disruption of mucolipin 1 function perturbs lysosomal homeostasis, promoting accumulation of undegraded substrates and altered mitochondrial quality control. Mcoln1 is therefore widely studied in models of lysosomal storage disorders and neurodegeneration-associated defects in endolysosomal function.
mucolipin 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mcoln1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mcoln1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mcoln1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish mucolipin 1 protein expression.
This CRISPR knockout system enables efficient generation of Mcoln1-deficient cell models for investigation of mucolipin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mcoln1 exon(s) critical for mucolipin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mcoln1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by mucolipin 1 CRISPR/Cas9 KO Plasmid (m) and mucolipin 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mcoln1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by mucolipin 1 HDR Plasmid (m) and mucolipin 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mcoln1 homology arms to support homology-directed repair at defined Mcoln1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.