Mucin 5B Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1201-1500 mapping near the C-terminus of Mucin 5B of human origin
Mucin 5B Antibody (A-3) is recommended for detection of Mucin 5B of human origin by WB, IP, IF and ELISA
Anti-Mucin 5B Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 5B (A-3): sc-393952.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. The Mucin 5B gene, which contains a 3′ cis-element, is one of the four mucin genes mapped to human chromosome 11p15. Although Mucin 5B is the prominent human gallbladder mucin, it is also expressed and secreted in the colon. In addition, Mucin 5B is expressed in non-inflammed middle ears and normal esophagus, and is upregulated by chronic inflammation and highly secreted in the diseased middle ear. Mucin 5B is abnormally expressed in gastric carcinomatous tissues. Its expression in gastric cancer cells is controlled by a highly active distal promoter, which is upregulated by protein kinase C and repressed under the influence of methylation. Mucous differentiation and expression of Mucin 5B is retinoic acid- (RA) or retinol-dependent. RA control of mucin gene is mediated by the retinoid acid receptor RARa and, to a lesser extent, by RARg. The correlation of mucin protein levels in human cervical mucous with the peak at midcycle suggests that mucin may be important in sperm transit to the uterus.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended starting dilution for Western blot for sc-393952: Mucin 5B (A-3) monoclonal antibody?
Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
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