Mucin 5B/MUC5B Antibody (A-3): sc-393952

Mucin 5B Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mucin 5B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Mucin 5B antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mucin 5B is a high molecular weight glycoprotein that plays a crucial role in protecting and lubricating epithelial surfaces, particularly in respiratory and gastrointestinal tracts. Mucin 5B′s structure, characterized by a mucin core protein with extensive O-linked glycosylation, influences mucus viscosity and gel-forming properties, maintaining mucosal surface barrier function and facilitating pathogen clearance. Located on human chromosome 11p15, Mucin 5B shows notable expression in gallbladder, colon, non-inflamed middle ear, and normal esophagus, with significant upregulation during chronic inflammation. Mucin 5B expression in gastric cancer is regulated by a distal promoter influenced by protein kinase C activity and methylation status. Retinoic acid regulation of Mucin 5B expression highlights its importance in mucous differentiation and potential role in reproductive processes, such as facilitating sperm transit to the uterus.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Mucin 5B/MUC5B Antibody (A-3) References:

1. Characterization of human mucin 5B gene expression in airway epithelium and the genomic clone of the amino-terminal and 5'-flanking region.  |  Chen, Y., et al. 2001. Am J Respir Cell Mol Biol. 25: 542-53. PMID: 11713095
2. Application of a novel human cervical mucin-based assay demonstrates the absence of increased mucinase activity in bacterial vaginosis.  |  Wiggins, R., et al. 2002. Int J STD AIDS. 13: 755-60. PMID: 12437895
3. The physicochemical properties of two mucoids from bovine cervical mucin.  |  GIBBONS, RA. and GLOVER, FA. 1959. Biochem J. 73: 217-25. PMID: 13827904
4. Chemical properties of two mucoids from bovine cervical mucin.  |  GIBBONS, RA. 1959. Biochem J. 73: 209-17. PMID: 13827905
5. Mucin 5B promoter polymorphism is associated with idiopathic pulmonary fibrosis but not with development of lung fibrosis in systemic sclerosis or sarcoidosis.  |  Stock, CJ., et al. 2013. Thorax. 68: 436-41. PMID: 23321605
6. DUSP28 links regulation of Mucin 5B and Mucin 16 to migration and survival of AsPC-1 human pancreatic cancer cells.  |  Lee, J., et al. 2016. Tumour Biol. 37: 12193-12202. PMID: 27230679
7. Structural studies of sialylated oligosaccharides of human midcycle cervical mucin.  |  Yurewicz, EC., et al. 1987. J Biol Chem. 262: 4733-9. PMID: 3558366
8. COVID-19 Survivor Patients Carrying the Rs35705950 Risk Allele in MUC5B Have Higher Plasma Levels of Mucin 5B.  |  García-Carmona, S., et al. 2022. Curr Issues Mol Biol. 44: 3283-3290. PMID: 35892712
9. Study of the primary structures of the peptide core of bovine estrus cervical mucin. Possible existence of small similar subunits.  |  Rao, KS. and Masson, PL. 1977. J Biol Chem. 252: 7788-95. PMID: 562341
10. Control of human cervical mucin glycosylation by endogenous fucosyl and sialyltransferases.  |  Scudder, PR. and Chantler, EN. 1982. Adv Exp Med Biol. 144: 265-7. PMID: 7080919

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mucin 5B/MUC5B Antibody (A-3) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Mucin 5B/MUC5B Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mucin 5B/MUC5B Antibody (A-3) Specifications

Alternate Product Name: MUC5B

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1201-1500

Epitope Target: Mucin 5B

Epitope Species: human

Product Reactivity/Detection: Mucin 5B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Mucin 5B/MUC5B Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Mucin 5B/MUC5B antibody is Mucin 5B/MUC5B Antibody (5B#19-2E): sc-21768.