Mucin 5B Antibody (5B#19-2E) recommended for detection of Mucin 5B of mouse, rat and human origin by WB, IP and IF

Mucin 5B Antibody (5B#19-2E): sc-21768

Mucin 5B Antibody (5B#19-2E) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-Mucin 5B Antibody (5B#19-2E) is a mouse monoclonal IgG1 (kappa light chain) Mucin 5B antibody provided at 200 µg/ml
    • raised against a peptide mapping at the N-terminus of Mucin 5B of human origin
    • Anti-Mucin 5B Antibody (5B#19-2E) is recommended for detection of Mucin 5B of mouse, rat and human origin by WB, IP and IF
    • Mucin 5B Antibody (5B#19-2E) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 5B (5B#19-2E): sc-21768.
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What is the recommended fixation method for immunofluorescence staining with Mucin 5B (5B#19-2E): sc-21768 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF on mouse lungs works great with this ab Immunofluorescence staining on mouse lungs works great with this ab.
Date published: 2018-09-19
Rated 5 out of 5 by from 一直在用一抗,因为停产,所以需要更换新的货号 一直在用一抗SC-20119,因为停产,所以需要更换新的货号Mucin 5B (5B#19-2E).
Date published: 2018-05-02
Rated 5 out of 5 by from Great staining A great antibody when in comes to IF and IHC. The signal is bright, clear, and specific. This antibody reacts well with mouse, rat, and human samples and has been used multiple times for publishing.
Date published: 2017-02-08
Rated 5 out of 5 by from Compliance statement Used to verify the mucin protein level in the cervical mucus and the peak at the central peak of the correlation, we purchased this product, product features consistent with the purchase instructions
Date published: 2017-01-18
Rated 5 out of 5 by from Worked for IF with methanol Worked for IF with methanol-fixed MCF7 cells. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Publication in IHC with human and mouse lung Publication in IHC with human and mouse lung samples. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good WB data with human tracheobronchial Good WB data with human tracheobronchial epithelial culture and human saliva. -SCBT QC
Date published: 2013-08-20
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