Mucin 5B/MUC5B Antibody (5B#19-2E): sc-21768

Mucin 5B Antibody (5B#19-2E) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mucin 5B (MUC5B) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-MUC5B antibody (5B#19-2E) is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MUC5B is a high molecular weight glycoprotein that plays a crucial role in forming protective mucus layers in various tissues, particularly in the respiratory and gastrointestinal tracts. MUC5B functions to trap pathogens and particulate matter, serving as a first line of defense against infections and maintaining mucosal integrity. MUC5B is predominantly expressed in the gallbladder and colon, and expression significantly increases in response to chronic inflammation, particularly in the middle ear, where secretion increases during disease states. MUC5B is also implicated in gastric cancer, where expression is regulated by a distal promoter influenced by protein kinase C activity and methylation status. MUC5B expression is further modulated by retinoic acid, which is essential for mucous differentiation, highlighting MUC5B′s importance in both normal physiology and disease processes.

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Mucin 5B/MUC5B Antibody (5B#19-2E) References:

1. The Amount of MUC5B mucin in cervical mucus peaks at midcycle.  |  Gipson, IK., et al. 2001. J Clin Endocrinol Metab. 86: 594-600. PMID: 11158014
2. Regulation of mucous differentiation and mucin gene expression in the tracheobronchial epithelium.  |  Gray, T., et al. 2001. Toxicology. 160: 35-46. PMID: 11246122
3. Aberrant expression of human mucin gene MUC5B in gastric carcinoma and cancer cells. Identification and regulation of a distal promoter.  |  Perrais, M., et al. 2001. J Biol Chem. 276: 15386-96. PMID: 11278696
4. Up-regulation of mucin secretion in HT29-MTX cells by the pro-inflammatory cytokines tumor necrosis factor-alpha and interleukin-6.  |  Smirnova, MG., et al. 2001. Eur Cytokine Netw. 12: 119-25. PMID: 11282555
5. MUC1 and MUC2 expression in human gallbladder carcinoma: a clinicopathological study and relationship with prognosis.  |  Kashiwagi, H., et al. 2001. Oncol Rep. 8: 485-9. PMID: 11295067
6. Characterization of mucins in human middle ear and Eustachian tube.  |  Lin, J., et al. 2001. Am J Physiol Lung Cell Mol Physiol. 280: L1157-67. PMID: 11350794
7. Mucin 5B promoter polymorphism is associated with idiopathic pulmonary fibrosis but not with development of lung fibrosis in systemic sclerosis or sarcoidosis.  |  Stock, CJ., et al. 2013. Thorax. 68: 436-41. PMID: 23321605
8. DUSP28 links regulation of Mucin 5B and Mucin 16 to migration and survival of AsPC-1 human pancreatic cancer cells.  |  Lee, J., et al. 2016. Tumour Biol. 37: 12193-12202. PMID: 27230679
9. Mucin 5B in saliva and serum of patients with oral lichen planus.  |  Agha-Hosseini, F., et al. 2017. Sci Rep. 7: 12060. PMID: 28935947
10. COVID-19 Survivor Patients Carrying the Rs35705950 Risk Allele in MUC5B Have Higher Plasma Levels of Mucin 5B.  |  García-Carmona, S., et al. 2022. Curr Issues Mol Biol. 44: 3283-3290. PMID: 35892712
11. Identification of a 42-kDa nuclear factor (NF1-MUC5B) from HT-29 MTX cells that binds to the 3' region of human mucin gene MUC5B.  |  Pigny, P., et al. 1996. Biochem Biophys Res Commun. 220: 186-91. PMID: 8602841
12. MUC5B is the prominent mucin in human gallbladder and is also expressed in a subset of colonic goblet cells.  |  van Klinken, BJ., et al. 1998. Am J Physiol. 274: G871-8. PMID: 9612268

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mucin 5B/MUC5B Antibody (5B#19-2E) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Mucin 5B/MUC5B Antibody (5B#19-2E) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mucin 5B/MUC5B Antibody (5B#19-2E) Specifications

Alternate Product Name: MUC5B

Product Clone Name: 5B#19-2E

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Mucin 5B

Epitope Species: human

Product Reactivity/Detection: Mucin 5B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Mucin 5B/MUC5B Antibody (5B#19-2E): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin