Mucin 5AC/MUC5AC Antibody (45M1): sc-21701

Mucin 5AC Antibody (45M1) is a mouse monoclonal IgG1 antibody that detects Mucin 5AC (MUC5AC) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-MUC5AC antibody (45M1) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MUC5AC is a crucial gel-forming mucin secreted by surface mucous cells, playing a vital role in protecting and lubricating epithelial surfaces, particularly in respiratory and gastrointestinal tracts. MUC5AC′s structure, characterized by a high molecular weight glycoprotein core with extensive O-linked glycosylation, influences mucin viscosity and ability to trap pathogens and particulates. MUC5AC synthesis is regulated by various factors, including glucocorticoids necessary for MUC5AC mRNA expression, while pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-alpha enhance secretion, linking MUC5AC to chronic inflammatory conditions. MUC5AC expression is also modulated by retinoic acid and thyroid hormones, highlighting complex regulatory mechanisms crucial for maintaining mucosal health. Anti-MUC5AC antibody (45M1) serves as an invaluable tool for researchers studying mucin biology, respiratory diseases, and inflammation effects on mucin expression.

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Mucin 5AC/MUC5AC Antibody (45M1) References:

1. Allergen-induced IL-9 directly stimulates mucin transcription in respiratory epithelial cells.  |  Longphre, M., et al. 1999. J Clin Invest. 104: 1375-82. PMID: 10562299
2. Expression of mucin genes in chronic ethmoiditis.  |  Jung, HH., et al. 2000. Am J Rhinol. 14: 163-70. PMID: 10887622
3. Mucin gene expression and cell differentiation in human normal, premalignant and malignant esophagus.  |  Guillem, P., et al. 2000. Int J Cancer. 88: 856-61. PMID: 11093805
4. Differential expression of MUC2 and MUC5AC in benign and malignant glandular lesions of the cervix uteri.  |  Riethdorf, L., et al. 2000. Virchows Arch. 437: 365-71. PMID: 11097361
5. Regulation of mucous differentiation and mucin gene expression in the tracheobronchial epithelium.  |  Gray, T., et al. 2001. Toxicology. 160: 35-46. PMID: 11246122
6. Up-regulation of mucin secretion in HT29-MTX cells by the pro-inflammatory cytokines tumor necrosis factor-alpha and interleukin-6.  |  Smirnova, MG., et al. 2001. Eur Cytokine Netw. 12: 119-25. PMID: 11282555
7. MUC1 and MUC2 expression in human gallbladder carcinoma: a clinicopathological study and relationship with prognosis.  |  Kashiwagi, H., et al. 2001. Oncol Rep. 8: 485-9. PMID: 11295067
8. Immunohistochemical demonstration of alpha1,4-N-acetylglucosaminyltransferase that forms GlcNAcalpha1,4Galbeta residues in human gastrointestinal mucosa.  |  Zhang, MX., et al. 2001. J Histochem Cytochem. 49: 587-96. PMID: 11304796
9. Effect of glucocorticoid on expression of rat MUC5AC mRNA in rat gastric mucosa in vivo and in vitro.  |  Tanaka, T., et al. 2001. Biol Pharm Bull. 24: 634-7. PMID: 11411550
10. Mucin 5AC Serves as the Nexus for β-Catenin/c-Myc Interplay to Promote Glutamine Dependency During Pancreatic Cancer Chemoresistance.  |  Ganguly, K., et al. 2022. Gastroenterology. 162: 253-268.e13. PMID: 34534538
11. Mucin 5AC-Mediated CD44/ITGB1 Clustering Mobilizes Adipose-Derived Mesenchymal Stem Cells to Modulate Pancreatic Cancer Stromal Heterogeneity.  |  Ganguly, K., et al. 2022. Gastroenterology. 162: 2032-2046.e12. PMID: 35219699
12. Mucin 5AC expression is common but unrelated to tumor progression in pancreatic adenocarcinoma.  |  Dwertmann Rico, S., et al. 2022. Int J Immunopathol Pharmacol. 36: 3946320221106504. PMID: 35764407

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mucin 5AC/MUC5AC Antibody (45M1) has 68 citations in a variety of scientific publications.

Primary antibodies like Anti-Mucin 5AC/MUC5AC Antibody (45M1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mucin 5AC/MUC5AC Antibody (45M1) Specifications

Alternate Product Name: MUC5AC

Product Clone Name: 45M1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Mucin 5AC

Epitope Species: human

Product Reactivity/Detection: Mucin 5AC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: rabbit, porcine, feline, avian, monkey and hedgehog

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for Mucin 5AC/MUC5AC Antibody (45M1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin