Mucin 4 Antibody (1G8) recommended for detection of Mucin 4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Mucin 4 Antibody (1G8): sc-33654

Mucin 4 Antibody (1G8) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-Mucin 4 Antibody (1G8) is a mouse monoclonal IgG1 (kappa light chain) Mucin 4 antibody provided at 200 µg/ml
    • raised against the transmembrane domain of Mucin 4 of rat Mucin 4
    • Anti-Mucin 4 Antibody (1G8) is recommended for detection of Mucin 4 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Mucin 4 Antibody (1G8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 4 (1G8): sc-33654.
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Can I use your conjugated Mucin 4 antibody for flow cytometry or sorting?

Asked by: Bartha
Thank you for your question. Yes, the conjugated form on this mouse monoclonal Mucin 4 (1G8) antibody, sc-33654, is recommended for Flow cytometry.
Answered by: Tech Service
Date published: 2020-01-31

Mucin 4 Antibody (1G8): sc-33654 Do you sell the blocking peptide? Thank you!

Asked by: Roxana Schillaci
Thank you for your question. No, we do not offer a binding protein for this monoclonal antibody. We suggest using normal mouse IgG as a negative control.
Answered by: Tech Service
Date published: 2019-01-16

I would like to use Mucin 4 Antibody (1G8): sc-33654 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-33654 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-30
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Rated 5 out of 5 by from Works in gerbils! Very pleased that this first Mucin 4 antibody I tried worked.
Date published: 2018-06-14
Rated 5 out of 5 by from Highly recommended for IHC(P) and WB. The go to monoclonal antibody for Mucin 4. Strong Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue. Well characterized in the primary literature.
Date published: 2017-01-07
Rated 5 out of 5 by from Publishable immunostaining in human paraffin Publishable immunostaining in human paraffin-embedded esphageal adenocarcinoma cells. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by from Great antibody for WB Great antibody for WB, works well in HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human duodenum. -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from Great mono Great mono! Successfully detects Mucin 4 activation in sc-400329-ACT transfected cells. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human gastric cancer cells. -SCBT Publication Review
Date published: 2015-01-02
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