Mucin 2 Antibody (Ccp58) recommended for detection of Mucin 2 of mouse, rat and human origin by IF and IHC(P)

Mucin 2 Antibody (Ccp58): sc-7314

Mucin 2 Antibody (Ccp58) is rated 4.1 out of 5 by 14.
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Datasheets

    • Mucin 2 Antibody (Ccp58) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a synthetic peptide encoding 5 tandem repeats of Mucin 2 of human origin
    • Anti-Mucin 2 Antibody (Ccp58) is recommended for detection of Mucin 2 of mouse, rat and human origin by IF and IHC(P)
    • Mucin 2 Antibody (Ccp58) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 2 (Ccp58): sc-7314.
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What concentration do you recommend for this antibody for mouse detection via IF and IHC?

Asked by: crtz
Thank you for your question. The recommended starting dilution is 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-09-27

Will this antibody work in a Sandwich Elisa?

Asked by: KathrinR
Thank you for your question. We have not tested this antibody in an ELISA and so this is not a recommended application at this time.
Answered by: Technical Support
Date published: 2019-04-26

This might be a novice question, however, shouldn't a primary antibody never be from the same host as the target species? Nonetheless, you suggest using this mouse monoclonal antibody to detect mucin 2 in mice. Could you elaborate how this works?

Asked by: Mwolter
Thank you for your question. There is no problem with using a mouse monoclonal antibody for detection of the corresponding protein in mouse cells or tissues. The main concern would be that your secondary antibody (anti-mouse IgG) may cross react non-specifically with endogenous mouse IgG expressed in your samples. This is only really an issue if your samples are rich in endogenous IgG. In that case, we would recommend optimizing your secondary antibody, blocking and incubation conditions. Alternatively, you can always use a directly conjugated primary antibody and avoid using any secondary antibody for detection.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody cross-react in my zebrafish samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13

What do you recommend for secondary detection in my Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 1 out of 5 by from Does not stain goblet cells in murine intestine IF We purchased two lots of this antibody and they do not stain for Mucin2, instead they appear to stain extracellular matrix or some other protein in the basal lamina propria (attached). We believe this is lot specific (attached), however customer service has chosen to ignore this after we politely brought it to their attention. We do not recommend purchasing this antibody, and are disappointed but not surprised by santa cruz's response to our concerns. We are however amused by the staff 5-star ratings of this antibody, fair play.
Date published: 2019-11-14
Rated 4 out of 5 by from 使用方便,功能齐全 我在一个月前购买了mucin-2 此产品,并用于黏液层免疫组化染色,实验效果显著,这个产品使用方便,且抗体效价很高。
Date published: 2019-06-08
Rated 1 out of 5 by from No effect on mouse colon IF staining No effect on colonic IF staining in mice, concentration 1:100
Date published: 2019-05-11
Rated 1 out of 5 by from Mouse small intestine tissue Doesn't work for mouse small intestine tissue via IF on cryosections & IF on mouse small intestinal organoids.
Date published: 2018-07-08
Rated 5 out of 5 by from strong band No non-specific binding and background ,just strong band,I am not recommend use Tween-20 when blocking the membrane,it may cause background.
Date published: 2017-07-01
Rated 5 out of 5 by from specific staining We use Mucin 2 antibody by Immunohistochemistry of human tonsil tissue stained with Anti-Mucin 2 antibody , we got the specific staining.
Date published: 2017-05-28
Rated 5 out of 5 by from satisfied data very outstanding antibody for Western blot. Strong signal obtained at dilution 1:500,satisfied result.
Date published: 2017-05-27
Rated 5 out of 5 by from Outstanding IHC staining Very nice product for IHC staining applications !!
Date published: 2017-01-23
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