Mucin 12/MUC12 Double Nickase Plasmid (h): sc-400505-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 12/MUC12 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mucin 12/MUC12 Double Nickase Plasmid (h) and Mucin 12/MUC12 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MUC12. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 12/MUC12 Antibody (B-9): sc-377269
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 12/MUC12 Double Nickase Plasmid (h) | sc-400505-NIC | 20 µg | $410.00 | |||
Mucin 12/MUC12 Double Nickase Plasmid (h2) | sc-400505-NIC-2 | 20 µg | $410.00 |
MUC12 encodes mucin 12, a membrane-associated mucin enriched on epithelial surfaces where its heavily O-glycosylated extracellular domain contributes to the protective glycocalyx, barrier integrity, and cell–cell interactions. Through effects on apical membrane architecture and mucin-dependent signaling crosstalk, MUC12 can influence epithelial differentiation programs, adhesion dynamics, and responses to inflammatory cues. Altered mucin expression patterns, including dysregulated MUC12, are frequently studied in the context of epithelial remodeling, chronic inflammation, and gastrointestinal disease biology. As a surface glycoprotein with extensive glycan modification, MUC12 is also relevant to investigations of glycosylation pathways and how glycocalyx composition modulates tissue homeostasis.
Mucin 12/MUC12 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MUC12 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MUC12. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MUC12 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MUC12-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.