Anti-Mucin 12 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) Mucin 12 antibody provided at 200 µg/ml
raised against amino acids 61-360 mapping near the N-terminus of Mucin 12 of human origin
Mucin 12 Antibody (B-9) is recommended for detection of Mucin 12 of human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Mucin 12 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 12 (B-9): sc-377269.
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Labware ships FedEx Ground free of charge to the contiguous US
Mucin 12 Antibody (B-9) is a high quality monoclonal Mucin 12 antibody (also designated MUC11 antibody, Mucin 11 antibody or MUC12 antibody) suitable for the detection of the Mucin 12 protein of human origin. Mucin 12 Antibody (B-9) is available as both the non-conjugated anti-Mucin 12 antibody form, as well as multiple conjugated forms of anti-Mucin 12 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Membrane-associated and secretory mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. The mucin family consists of Mucins 1-4, Mucin 5 (AC and B), Mucins 6-8, Mucins 11-13 and Mucins 15-17. A family of four related Mucin genes (MUC2, MUC5AC, MUC5B and MUC6) encode the major secreted mucins. Mucin 12 contains a predicted transmembrane domain; two extracellular cysteine-rich EGF-like domains; a coiled-coil region; and a domain consisting of serine-, threonine-, and proline-rich degenerate tandem repeats of 28 amino acids, a structural feature typical of mucins. Mucin 12 transcript (>12 kb) is present in colon, pancreas, prostate and uterus. Colorectal tumors can have low Mucin 12 transcript levels in comparison to normal colon tissues.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can Mucin 12 (B-9): sc-377269 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: Dr Ninau Qelp
Thank you for your question. Yes, Mucin 12 (B-9): sc-377269 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 4 out of
Florencia Chervo from
Worked fine for WBWe tried a sample of this antibody (dilution 1:500 in PBS-tween 0.1%) for Western Blot and worked fine. We used BT-474 cells control (C) or overexpressing TNF-alpha (T2).
Date published: 2017-02-06
Rated 5 out of
Robust cytoplasmic staining in paraffinRobust cytoplasmic staining in paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-05-16
Rated 5 out of
Works well for Western with human rectumWorks well for Western with human rectum tissue extract. -SCBT QC
Date published: 2015-01-07
Rated 5 out of
Good specificity and signal strength in MESGood specificity and signal strength in MES-SA/Dx5 whole cell lysate. -SCBT QC
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