Date published: 2025-9-30

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Mucin 1/MUC1 Antibody (SM3): sc-53381

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Datasheets
  • Mucin 1/MUC1 Antibody (SM3) is a mouse monoclonal IgG1 λ, cited in 11 publications, provided at 200 µg/ml
  • raised against hydrogen fluoride deglycosylated milk mucin of human origin
  • Anti-Mucin 1/MUC1 Antibody (SM3) is recommended for detection of Mucin 1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • Anti-Mucin 1/MUC1 Antibody (SM3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Mucin 1/MUC1 Antibody (SM3) for WB and IHC(P) applications. This reagent is now offered in a bundle with Mucin 1/MUC1 Antibody (SM3) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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SEE ALSO...

Mucin 1 Antibody (SM3) is a mouse monoclonal IgG1 antibody that detects Mucin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Mucin 1 antibody (SM3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mucin 1 plays a crucial role in cell signaling and adhesion, acting as a protective barrier on epithelial surfaces and influencing tumor progression. Mucin 1 expression is particularly significant in epithelial tumors, such as breast carcinomas, where Mucin 1 can contribute to tumorigenesis and metastasis. Mucin 1 is primarily localized to the apical surface of epithelial cells, where Mucin 1 forms a protective mucosal layer, which is essential for maintaining tissue integrity and function. Understanding the localization and function of Mucin 1 is vital for developing targeted therapies in cancer treatment, as Mucin 1 overexpression can serve as a biomarker for malignancy.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Mucin 1/MUC1 Antibody (SM3)

sc-53381
200 µg/ml
$316.00

Mucin 1/MUC1 Antibody (SM3): m-IgGλ BP-HRP Bundle

sc-520974
200 µg Ab, 40 µg BP
$354.00

Mucin 1/MUC1 Antibody (SM3) AC

sc-53381 AC
500 µg/ml, 25% agarose
$416.00

Mucin 1/MUC1 Antibody (SM3) HRP

sc-53381 HRP
200 µg/ml
$316.00

Mucin 1/MUC1 Antibody (SM3) FITC

sc-53381 FITC
200 µg/ml
$330.00

Mucin 1/MUC1 Antibody (SM3) PE

sc-53381 PE
200 µg/ml
$343.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 488

sc-53381 AF488
200 µg/ml
$357.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 546

sc-53381 AF546
200 µg/ml
$357.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 594

sc-53381 AF594
200 µg/ml
$357.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 647

sc-53381 AF647
200 µg/ml
$357.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 680

sc-53381 AF680
200 µg/ml
$357.00

Mucin 1/MUC1 Antibody (SM3) Alexa Fluor® 790

sc-53381 AF790
200 µg/ml
$357.00

Do you know if sequence targeted by the antibodie is intra or extracellular?

Asked by: LilaGa
Thank you for your question. This mouse monoclonal antibody, Mucin 1 (SM3): sc-53381, is raised against hydrogen fluoride deglycosylated milk mucin of human origin. The exact epitope recognized by the antibody has not yet been determined, and therefore we do not know if the epitope is intracellular or extracellular.
Answered by: Technical Support
Date published: 2017-10-30

What is the molecular weight of Mucin 1 ?

Asked by: Trav11
Thank you for your question. Mucin 1 has an expected molecular weight of approximately 200 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from It doesn't work in mouse colon (Western Blot)Dilution tested 1:500 in mouse tissue estract. It doen't work.
Date published: 2020-10-07
Rated 4 out of 5 by from 特异性较好之前申请的试用装,结果显示,该抗体能特异性识别相应抗原,条带较清晰,适用于Western blot 检测。
Date published: 2018-04-24
Rated 5 out of 5 by from 清晰的目标条带在胰腺癌细胞株中使用了这支抗体,有清晰的目的条带,未见杂带,背景清晰,使用浓度1:500,总体上是一支合适wb的抗体
Date published: 2018-04-02
Rated 5 out of 5 by from Vimentin (C-20)-R antibodyI was kindly given this antibody by Santa Cruz to try on my current project.
Date published: 2018-03-07
Rated 5 out of 5 by from five starWorked well for Western Blotting in our validation of detection of Mucin 1 (mouse 1:500 dilution).
Date published: 2017-06-01
Rated 2 out of 5 by from Not cross-reactivity with bovineWe have tried the antibody on cells isolated from bovine milk and we were unable to detect any binding; thus, we concluded that the antibody does not work on the extracellular region of bovine MUC1.
Date published: 2017-05-22
Rated 5 out of 5 by from Great for WBThe Mucin 1 Antibody (SM3): sc-53381 provides clear band with minimal background in Western blot application.
Date published: 2017-01-19
Rated 5 out of 5 by from Single strong band in DU 145 whole cell lysateSingle strong band in DU 145 whole cell lysate. -SCBT QC
Date published: 2015-02-03
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Mucin 1/MUC1 Antibody (SM3) is rated 4.1 out of 5 by 9.
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