Mucin 1 Antibody (SM3) recommended for detection of Mucin 1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Mucin 1 Antibody (SM3): sc-53381

Mucin 1 Antibody (SM3) is rated 4.5 out of 5 by 8.
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Datasheets

    • Mucin 1 Antibody (SM3) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • raised against hydrogen fluoride deglycosylated milk mucin of human origin
    • Anti-Mucin 1 Antibody (SM3) is recommended for detection of Mucin 1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Mucin 1 Antibody (SM3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mucin 1 (SM3): sc-53381.
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Do you know if sequence targeted by the antibodie is intra or extracellular?

Asked by: LilaGa
Thank you for your question. This mouse monoclonal antibody, Mucin 1 (SM3): sc-53381, is raised against hydrogen fluoride deglycosylated milk mucin of human origin. The exact epitope recognized by the antibody has not yet been determined, and therefore we do not know if the epitope is intracellular or extracellular.
Answered by: Technical Support
Date published: 2017-10-30

What is the molecular weight of Mucin 1 ?

Asked by: Trav11
Thank you for your question. Mucin 1 has an expected molecular weight of approximately 200 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from 特异性较好 之前申请的试用装,结果显示,该抗体能特异性识别相应抗原,条带较清晰,适用于Western blot 检测。
Date published: 2018-04-24
Rated 5 out of 5 by from 清晰的目标条带 在胰腺癌细胞株中使用了这支抗体,有清晰的目的条带,未见杂带,背景清晰,使用浓度1:500,总体上是一支合适wb的抗体
Date published: 2018-04-02
Rated 5 out of 5 by from Vimentin (C-20)-R antibody I was kindly given this antibody by Santa Cruz to try on my current project.
Date published: 2018-03-07
Rated 5 out of 5 by from five star Worked well for Western Blotting in our validation of detection of Mucin 1 (mouse 1:500 dilution).
Date published: 2017-06-01
Rated 2 out of 5 by from Not cross-reactivity with bovine We have tried the antibody on cells isolated from bovine milk and we were unable to detect any binding; thus, we concluded that the antibody does not work on the extracellular region of bovine MUC1.
Date published: 2017-05-22
Rated 5 out of 5 by from Great for WB The Mucin 1 Antibody (SM3): sc-53381 provides clear band with minimal background in Western blot application.
Date published: 2017-01-19
Rated 5 out of 5 by from Single strong band in DU 145 whole cell lysate Single strong band in DU 145 whole cell lysate. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from WB: Works well in BT WB: Works well in BT-20 whole cell lysate. -SCBT QC
Date published: 2013-07-19
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