MTMR7 CRISPR/Cas9 KO Plasmid (h): sc-405863
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MTMR7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MTMR7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTMR7 CRISPR/Cas9 KO Plasmid (h) | sc-405863 | 20 µg | $397.00 |
Overview
MTMR7 encodes myotubularin related protein 7, a catalytically active lipid phosphatase that dephosphorylates phosphatidylinositol 3-phosphate and phosphatidylinositol (3,5)-bisphosphate, thereby tuning phosphoinositide-dependent membrane trafficking. Through regulation of endosomal dynamics, receptor recycling, and vesicle maturation, MTMR7 contributes to control of signaling outputs from pathways such as EGFR/MAPK and PI3K-associated networks. Altered MTMR7 activity has been linked to dysregulated cell proliferation and migration phenotypes, supporting its relevance in studies of oncogenic signaling and epithelial homeostasis. Its role in phosphoinositide turnover also intersects with broader processes including autophagy and lysosome function that are frequently perturbed in disease-associated cellular states.
MTMR7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MTMR7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MTMR7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MTMR7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MTMR7 protein expression.
This CRISPR knockout system enables efficient generation of MTMR7-deficient cell models for investigation of MTMR7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MTMR7 exon(s) critical for MTMR7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MTMR7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MTMR7 CRISPR/Cas9 KO Plasmid (h) and MTMR7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MTMR7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MTMR7 HDR Plasmid (h) and MTMR7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MTMR7 homology arms to support homology-directed repair at defined MTMR7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.