MTHFD1 Antibody (A-8): sc-271412

MTHFD1 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects MTHFD1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MTHFD1 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) is a crucial 935-amino acid, folate-dependent enzyme that plays a vital role in the interconversion of tetrahydrofolate derivatives, which are essential for the synthesis of purines, methionine, and thymidylate. MTHFD1 is located in the cytosol and exists as a homodimer composed of three distinct subunits: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, and 10-formyltetrahydrofolate synthetase, each contributing unique enzymatic activities. MTHFD1′s structural organization, with its N-terminal domain responsible for dehydrogenase and cyclohydrolase activities and a larger C-terminal synthetase domain, is critical for its function in folate metabolism. Mutations in MTHFD1 gene in pregnant women have been linked to an increased risk of neural tube defects in offspring, highlighting MTHFD1′s significance in developmental biology. MTHFD1 is also implicated in migraine pathophysiology, particularly in cases of migraine with aura, due to MTHFD1′s role in folate metabolism.

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MTHFD1 Antibody (A-8) References:

1. Role of polymorphisms in MTHFR and MTHFD1 genes in the outcome of childhood acute lymphoblastic leukemia.  |  Krajinovic, M., et al. 2004. Pharmacogenomics J. 4: 66-72. PMID: 14647408
2. Disruption of the mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria.  |  Christensen, KE., et al. 2005. J Biol Chem. 280: 7597-602. PMID: 15611115
3. MTHFD1 R653Q polymorphism is a maternal genetic risk factor for severe abruptio placentae.  |  Parle-McDermott, A., et al. 2005. Am J Med Genet A. 132A: 365-8. PMID: 15633187
4. Folate-related genes and omphalocele.  |  Mills, JL., et al. 2005. Am J Med Genet A. 136: 8-11. PMID: 15937947
5. Thymidylate synthase promoter tandem repeat and MTHFD1 R653Q polymorphisms modulate the risk for migraine conferred by the MTHFR T677 allele.  |  Oterino, A., et al. 2005. Brain Res Mol Brain Res. 139: 163-8. PMID: 15953655
6. A polymorphism in the MTHFD1 gene increases a mother's risk of having an unexplained second trimester pregnancy loss.  |  Parle-McDermott, A., et al. 2005. Mol Hum Reprod. 11: 477-80. PMID: 16123074
7. Confirmation of the R653Q polymorphism of the trifunctional C1-synthase enzyme as a maternal risk for neural tube defects in the Irish population.  |  Parle-McDermott, A., et al. 2006. Eur J Hum Genet. 14: 768-72. PMID: 16552426
8. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation.  |  Sdelci, S., et al. 2019. Nat Genet. 51: 990-998. PMID: 31133746
9. Arginine methylation of MTHFD1 by PRMT5 enhances anoikis resistance and cancer metastasis.  |  Meng, Q., et al. 2022. Oncogene. 41: 3912-3924. PMID: 35798877
10. Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells.  |  Green, AC., et al. 2023. Nat Metab. 5: 642-659. PMID: 37012496
11. MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma.  |  Guan, J., et al. 2024. Cell Death Dis. 15: 124. PMID: 38336749
12. Congenital defects in folate utilization.  |  Arakawa, T. 1970. Am J Med. 48: 594-8. PMID: 5425237

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTHFD1 Antibody (A-8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MTHFD1 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTHFD1 Antibody (A-8) Specifications

Alternate Product Name: MTHFD1

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-120

Epitope Target: MTHFD1

Epitope Species: human

Product Reactivity/Detection: MTHFD1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MTHFD1 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin