MTHFD1 Antibody (A-1): sc-271444

MTHFD1 Antibody (A-1) is a mouse monoclonal IgG2a antibody that detects methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MTHFD1 is a crucial 935-amino acid, folate-dependent enzyme that facilitates the interconversion of tetrahydrofolate derivatives, which are essential for the synthesis of purines, methionine, and thymidylate, thereby playing a vital role in cellular metabolism and DNA synthesis. MTHFD1 is located in the cytosol and exists as a homodimer composed of three distinct subunits: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, and 10-formyltetrahydrofolate synthetase, each contributing unique enzymatic activities. The structural organization of MTHFD1, with its N-terminal domain responsible for dehydrogenase and cyclohydrolase activities and a larger C-terminal synthetase domain, is critical for its function, as this allows for efficient catalysis and regulation of folate metabolism. Mutations in the MTHFD1 gene have been linked to an increased risk of neural tube defects in offspring of pregnant women, highlighting MTHFD1′s significance in developmental biology and maternal health. MTHFD1′s involvement in folate metabolism is associated with migraine pathophysiology, particularly in cases of migraine with aura, underscoring MTHFD1′s relevance in both metabolic and neurological contexts.

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MTHFD1 Antibody (A-1) References:

1. Mthfd1 is a modifier of chemically induced intestinal carcinogenesis.  |  MacFarlane, AJ., et al. 2011. Carcinogenesis. 32: 427-33. PMID: 21156972
2. Reduced MTHFD1 activity in male mice perturbs folate- and choline-dependent one-carbon metabolism as well as transsulfuration.  |  Field, MS., et al. 2013. J Nutr. 143: 41-5. PMID: 23190757
3. Association study of MTHFD1 coding polymorphisms R134K and R653Q with migraine susceptibility.  |  Sutherland, HG., et al. 2014. Headache. 54: 1506-14. PMID: 25039261
4. Association between MTHFD1 polymorphisms and neural tube defect susceptibility.  |  Meng, J., et al. 2015. J Neurol Sci. 348: 188-94. PMID: 25524527
5. Characterization and review of MTHFD1 deficiency: four new patients, cellular delineation and response to folic and folinic acid treatment.  |  Burda, P., et al. 2015. J Inherit Metab Dis. 38: 863-72. PMID: 25633902
6. Biochemical analysis of patients with mutations in MTHFD1 and a diagnosis of methylenetetrahydrofolate dehydrogenase 1 deficiency.  |  Bidla, G., et al. 2020. Mol Genet Metab. 130: 179-182. PMID: 32414565
7. A redox probe screens MTHFD1 as a determinant of gemcitabine chemoresistance in cholangiocarcinoma.  |  Pan, R., et al. 2021. Cell Death Discov. 7: 89. PMID: 33934113
8. Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.  |  Anderson, DE., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34544865
9. Association of MTHFD1 gene polymorphisms and maternal smoking with risk of congenital heart disease: a hospital-based case-control study.  |  Song, X., et al. 2022. BMC Pregnancy Childbirth. 22: 88. PMID: 35100977
10. Association of MTHFD1 G1958A Polymorphism with Gestational Diabetes Mellitus.  |  Bunga, PK., et al. 2024. Cureus. 16: e53287. PMID: 38435941

The preferred MTHFD1 antibody is MTHFD1 Antibody (A-8): sc-271412.