Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MTBP Double Nickase Plasmid (h): sc-404307-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MTBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MTBP Double Nickase Plasmid (h) and MTBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MTBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MTBP Antibody (B-5): sc-137201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTBP Double Nickase Plasmid (h) | sc-404307-NIC | 20 µg | $410.00 | |||
MTBP Double Nickase Plasmid (h2) | sc-404307-NIC-2 | 20 µg | $410.00 |
Mdm2 binding protein (MTBP) is a nuclear factor implicated in control of DNA replication initiation and S-phase progression through interactions with replication proteins such as Treslin/TICRR and CDC45, supporting origin firing and genome stability. MTBP also interfaces with cell-cycle checkpoint and stress-response signaling, linking replication dynamics to proliferation control. Dysregulated MTBP expression has been associated with altered replication stress tolerance and chromosomal instability observed across multiple tumor contexts, making it a useful locus for studying pathways that couple DNA replication with oncogenic growth programs. Functional interrogation of MTBP informs mechanisms governing replication timing, DNA damage signaling, and mitotic fidelity in human cells.
MTBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MTBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MTBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MTBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MTBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.