MSMP CRISPR/Cas9 KO Plasmid (h): sc-418716
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MSMP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MSMP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSMP CRISPR/Cas9 KO Plasmid (h) | sc-418716 | 20 µg | $397.00 |
Overview
MSMP (microseminoprotein, beta) encodes a small secreted protein with enriched expression in prostate epithelium and detectability in extracellular fluids, supporting a role in local paracrine signaling within the male reproductive tract. Although its precise receptor interactions remain incompletely defined, MSMP has been linked to regulation of epithelial differentiation, secretory homeostasis, and tumor–stroma communication, processes that intersect with androgen-responsive transcriptional programs. Altered MSMP expression has been reported across prostate cancer molecular subtypes and is frequently studied alongside inflammatory and microenvironmental remodeling pathways that influence progression. These features make MSMP a useful marker and functional node for interrogating secreted-factor biology in prostate-derived models.
MSMP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MSMP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MSMP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MSMP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MSMP protein expression.
This CRISPR knockout system enables efficient generation of MSMP-deficient cell models for investigation of MSMP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MSMP exon(s) critical for MSMP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MSMP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MSMP CRISPR/Cas9 KO Plasmid (h) and MSMP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MSMP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MSMP HDR Plasmid (h) and MSMP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MSMP homology arms to support homology-directed repair at defined MSMP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.