MSH2 Antibody (D-9): sc-515356
- MSH2 Antibody (D-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 10-33 near the N-terminus of MSH2 of human origin
- Anti-MSH2 Antibody (D-9) is recommended for detection of MSH2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MSH2 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MSH2 Antibody (D-9) for WB applications. These reagents are now offered in bundles with MSH2 Antibody (D-9) (see ordering information below).
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MSH2 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects MSH2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MSH2 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MSH2 protein plays a critical role in the DNA mismatch repair (MMR) system, which is essential for maintaining genomic stability by correcting errors that occur during DNA replication. Deficiencies in this repair mechanism can lead to an accumulation of mutations, significantly increasing the risk of various cancers, including hereditary nonpolyposis colorectal cancer (HNPCC). Notably, mutations in MSH2 and MSH2 homolog MLH1 have been linked to a high frequency of microsatellite instability, a hallmark of many tumors. Furthermore, the presence of microsatellite instability in a range of cancers, such as pancreatic, gastric, breast, ovarian, and small cell lung cancers, underscores MSH2′s importance in tumorigenesis beyond hereditary conditions. The identification of additional homologs, PMS1 and PMS2, which are also implicated in MMR and associated with HNPCC, highlights the complexity and significance of this pathway in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MSH2 Antibody (D-9) References:
- Msh2 separation of function mutations confer defects in the initiation steps of mismatch repair. | Kijas, AW., et al. 2003. J Mol Biol. 331: 123-38. PMID: 12875840
- Msh2 deficiency leads to chromosomal abnormalities, centrosome amplification, and telomere capping defect. | Campbell, MR., et al. 2006. Oncogene. 25: 2531-6. PMID: 16331258
- MSH2 deficiency abolishes the anticancer and pro-aging activity of short telomeres. | Martinez, P., et al. 2009. Aging Cell. 8: 2-17. PMID: 18986375
- Msh2 deficiency leads to dysmyelination of the corpus callosum, impaired locomotion, and altered sensory function in mice. | Diouf, B., et al. 2016. Sci Rep. 6: 30757. PMID: 27476972
- Analysis of MSH2 Loss of Heterozygosity, Expression, and IVS10+12G>A Polymorphism in Sporadic Colon Cancer. | Cacev, T., et al. 2018. Anticancer Res. 38: 2841-2848. PMID: 29715107
- Prerecognition Diffusion Mechanism of Human DNA Mismatch Repair Proteins along DNA: Msh2-Msh3 versus Msh2-Msh6. | Pal, A., et al. 2020. Biochemistry. 59: 4822-4832. PMID: 33319999
- Multiplexed assays reveal effects of missense variants in MSH2 and cancer predisposition. | Nielsen, SV., et al. 2021. PLoS Genet. 17: e1009496. PMID: 33886538
- circEPSTI1 promotes tumor progression and cisplatin resistance via upregulating MSH2 in cervical cancer. | Wu, P., et al. 2022. Aging (Albany NY). 14: 5406-5416. PMID: 35779530
- CircWDR26 regulates endometrial carcinoma progression via miR-212-3p-mediated typing genes MSH2. | Lei, TX., et al. 2022. Eur J Med Res. 27: 135. PMID: 35897048
- Hesperetin effect on MLH1 and MSH2 expression on breast cancer cells BT-549. | Hasan, AK., et al. 2023. J Adv Pharm Technol Res. 14: 241-247. PMID: 37692022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSH2 Antibody (D-9) | sc-515356 | 200 µg/ml | $322.00 | |||
MSH2 Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-528027 | 200 µg Ab; 10 µg BP | $361.00 | |||
MSH2 Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-533400 | 200 µg Ab; 20 µg BP | $361.00 | |||
MSH2 Antibody (D-9) AC | sc-515356 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MSH2 Antibody (D-9) HRP | sc-515356 HRP | 200 µg/ml | $322.00 | |||
MSH2 Antibody (D-9) FITC | sc-515356 FITC | 200 µg/ml | $336.00 | |||
MSH2 Antibody (D-9) PE | sc-515356 PE | 200 µg/ml | $349.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 488 | sc-515356 AF488 | 200 µg/ml | $364.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 546 | sc-515356 AF546 | 200 µg/ml | $364.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 594 | sc-515356 AF594 | 200 µg/ml | $364.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 647 | sc-515356 AF647 | 200 µg/ml | $364.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 680 | sc-515356 AF680 | 200 µg/ml | $364.00 | |||
MSH2 Antibody (D-9) Alexa Fluor® 790 | sc-515356 AF790 | 200 µg/ml | $364.00 | |||
MSH2 (D-9) Neutralizing Peptide | sc-515356 P | 100 µg/0.5 ml | $69.00 |