Date published: 2026-4-15
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MSH2 Antibody (D-6) - Western Blotting - Image 286123
MSH2 Antibody (D-6): sc-376384. Western blot analysis of MSH2 expression in Hep G2 (A), Caki-1 (B) and EOC 20 (C) whole cell lysates.
MSH2 Antibody (D-6): sc-376384. Western blot analysis of MSH2 expression in Hep G2 (A), Caki-1 (B) and EOC 20 (C) whole cell lysates.

MSH2 Antibody (D-6): sc-376384

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Datasheets
  • MSH2 Antibody (D-6) is a mouse monoclonal IgG1 κ MSH2 antibody, cited in 5 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 of MSH2 of human origin
  • MSH2 Antibody (D-6) is recommended for detection of MSH2 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-MSH2 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for MSH2 Antibody (D-6) for WB applications. This reagent is now offered in a bundle with MSH2 Antibody (D-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Additional MSH Antibodies including MSH3

MSH2 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MSH2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MSH2 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MSH2 plays a critical role in the DNA mismatch repair (MMR) system, which is essential for maintaining genomic stability by correcting errors that occur during DNA replication. Deficiencies in this repair mechanism can lead to microsatellite instability, a hallmark of several cancers, including hereditary nonpolyposis colorectal cancer (HNPCC). MSH2′s importance extends beyond HNPCC, as studies have shown that 10 to 45% of various cancers, such as pancreatic, gastric, breast, ovarian, and small cell lung cancers, also exhibit microsatellite instability, indicating that MMR dysfunction may be a common feature in tumor initiation and progression. Additionally, the identification of additional homologs, PMS1 and PMS2, which are also implicated in MMR and mutated in HNPCC patients, underscores MSH2′s significance in cancer biology and highlights MSH2′s potential as a target for therapeutic intervention and diagnostic applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

MSH2 Antibody (D-6) References:

  1. HDAC6 deacetylates and ubiquitinates MSH2 to maintain proper levels of MutSα.  |  Zhang, M., et al. 2014. Mol Cell. 55: 31-46. PMID: 24882211
  2. MSH2 Dysregulation Is Triggered by Proinflammatory Cytokine Stimulation and Is Associated with Liver Cancer Development.  |  Eso, Y., et al. 2016. Cancer Res. 76: 4383-93. PMID: 27261510
  3. Clinical significance of MLH1/MSH2 for stage II/III sporadic colorectal cancer.  |  Wang, SM., et al. 2019. World J Gastrointest Oncol. 11: 1065-1080. PMID: 31798786
  4. Insertion of an SVA element in MSH2 as a novel cause of Lynch syndrome.  |  Yang, C., et al. 2021. Genes Chromosomes Cancer. 60: 571-576. PMID: 33822432
  5. Cytoplasmic MSH2 Related to Genomic Deletions in the MSH2/EPCAM Genes in Colorectal Cancer Patients With Suspected Lynch Syndrome.  |  Dong, L., et al. 2021. Front Oncol. 11: 627460. PMID: 34055602
  6. Analysis of the Expression and Prognostic Value of MSH2 in Pan-Cancer Based on Bioinformatics.  |  Qiu, W., et al. 2021. Biomed Res Int. 2021: 9485273. PMID: 34859104
  7. Andrias davidianus Ranavirus (ADRV) Genome Replicate Efficiently by Engaging Cellular Mismatch Repair Protein MSH2.  |  Ke, F., et al. 2022. Viruses. 14: PMID: 35632694
  8. Ataxia Telangiectasia Mutated and MSH2 Control Blunt DNA End Joining in Ig Class Switch Recombination.  |  Sible, E., et al. 2023. J Immunol. 210: 369-376. PMID: 36603026
  9. Protein profiles reveal MSH6/MSH2 as a potential biomarker for hepatocellular carcinoma with microvascular invasion.  |  Hong, S., et al. 2024. Hepatol Res. 54: 189-200. PMID: 37776019
  10. Muir-Torre Syndrome with Novel Mutation in the MSH2 Gene.  |  Ustaoglu, E., et al. 2023. Acta Dermatovenerol Croat. 31: 144-147. PMID: 38439724

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

MSH2 Antibody (D-6)

sc-376384
200 µg/ml
$322.00

MSH2 Antibody (D-6): m-IgGκ BP-HRP Bundle

sc-522938
200 µg Ab, 40 µg BP
$361.00

MSH2 Antibody (D-6) AC

sc-376384 AC
500 µg/ml, 25% agarose
$424.00

MSH2 Antibody (D-6) HRP

sc-376384 HRP
200 µg/ml
$322.00

MSH2 Antibody (D-6) FITC

sc-376384 FITC
200 µg/ml
$336.00

MSH2 Antibody (D-6) PE

sc-376384 PE
200 µg/ml
$349.00

MSH2 Antibody (D-6) Alexa Fluor® 488

sc-376384 AF488
200 µg/ml
$364.00

MSH2 Antibody (D-6) Alexa Fluor® 546

sc-376384 AF546
200 µg/ml
$364.00

MSH2 Antibody (D-6) Alexa Fluor® 594

sc-376384 AF594
200 µg/ml
$364.00

MSH2 Antibody (D-6) Alexa Fluor® 647

sc-376384 AF647
200 µg/ml
$364.00

MSH2 Antibody (D-6) Alexa Fluor® 680

sc-376384 AF680
200 µg/ml
$364.00

MSH2 Antibody (D-6) Alexa Fluor® 790

sc-376384 AF790
200 µg/ml
$364.00

I know D-6 is the replacement of N-20. Could you tell me does they have the same immunogen amino acid sequences? Their applications look like the same.

Asked by: JamesChou
Thank you for your question. The two antibodies have overlapping immunogen sequences, but they are not the same. This new mouse monoclonal antibody, MSH2 (D-6): sc-376384, is raised against a recombinant protein corresponding to amino acids 1-300 of MSH2 of human origin. The rabbit polyclonal antibody, MSH2 (N-20): sc-494, was raised against a short peptide sequence mapping at the N-terminus of MSH2 of human origin. We do offer a different monoclonal antibody, MSH2 (E-7): sc-365052, which is raised against the same peptide used to generate the rabbit polyclonal (N-20) antibody.
Answered by: Technical Support
Date published: 2017-10-05

What is the recommended fixation method for immunofluorescence staining with MSH2 (D-6): sc-376384 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good for WBWe used this antibody in U2OS and E14 cells. It is very strong and clear.
Date published: 2018-02-20
Rated 4 out of 5 by from Works great for Western BlottingI ordered a free sample to test this one out few weeks ago in human corneal endothelial cells. It gave a strong signal when used at 1:500 dilution in 5% milk+ TBS-tween and developed with mouse-IgGκ BP-HRP(SCBT) at 1:1000 dilution in 5% milk+ TBS-tween.
Date published: 2018-02-02
Rated 5 out of 5 by from Worked in Western blotworks well in Western blot of Human Hek293 cells. Block: 5%milk PBST primary antibody: 1/500 in 1% milk PBST cold room overnight secondary antibody: 1/10,000 anti-mouse HRP in 1% milk PBST ECL Dura substrate 5 minutes
Date published: 2017-11-29
Rated 5 out of 5 by from Good antibodyI requested this free antibody sample a few weeks ago, it works in my Hela samples at 1:100 dilution, blocked by 5% non-fat milk, but has some other bands below
Date published: 2017-10-03
Rated 5 out of 5 by from good for WBI used this antiboty 1:1000 for WB, the band is clear and have no background
Date published: 2017-09-23
Rated 3 out of 5 by from okThis antibody worked well for my WB by using human keratinocyte.
Date published: 2017-02-09
Rated 5 out of 5 by from Versatile Antibody, Strong Signal in WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-03
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~100 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, and weak detection in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
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MSH2 Antibody (D-6) is rated 4.7 out of 5 by 11.
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