MRS2L CRISPR/Cas9 KO Plasmid (h): sc-410061
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRS2L CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRS2L genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRS2L CRISPR/Cas9 KO Plasmid (h) | sc-410061 | 20 µg | $397.00 |
Overview
Human MRS2 encodes MRS2L, a mitochondrial inner membrane Mg2+ channel essential for maintaining matrix magnesium homeostasis, supporting oxidative phosphorylation, and buffering bioenergetic stress. By regulating Mg2+-dependent activities such as ATP handling and mitochondrial enzyme function, MRS2L influences mitochondrial membrane potential, calcium coupling, and reactive oxygen species balance. Perturbation of mitochondrial ion transport and Mg2+ availability is implicated in defects in mitochondrial metabolism and stress responses that are frequently studied in neurodegeneration, cardiometabolic disorders, and cancer cell adaptation. MRS2L therefore serves as a useful node for interrogating mitochondrial signaling, metabolic plasticity, and organelle quality control pathways.
MRS2L CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MRS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MRS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MRS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRS2L protein expression.
This CRISPR knockout system enables efficient generation of MRS2-deficient cell models for investigation of MRS2L signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MRS2 exon(s) critical for MRS2L function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MRS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRS2L CRISPR/Cas9 KO Plasmid (h) and MRS2L CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MRS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRS2L HDR Plasmid (h) and MRS2L HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MRS2 homology arms to support homology-directed repair at defined MRS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.