MRP6 Double Nickase Plasmid (h): sc-402219-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP6 Double Nickase Plasmid (h) and MRP6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCC6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP6 Antibody (M6II-31): sc-59618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP6 Double Nickase Plasmid (h) | sc-402219-NIC | 20 µg | $410.00 |
ABCC6 encodes the human ATP-binding cassette transporter MRP6, a membrane efflux pump primarily linked to regulation of extracellular nucleotide and pyrophosphate homeostasis. By influencing ATP release and downstream conversion to inorganic pyrophosphate, MRP6 helps modulate mineralization pathways and extracellular matrix remodeling. Altered ABCC6 activity is strongly associated with ectopic calcification phenotypes and connective tissue pathology, including pseudoxanthoma elasticum, and has been investigated in vascular and dermal biology contexts. These functions connect ABCC6 to broader purinergic signaling, mineral metabolism, and transporter-mediated control of tissue microenvironments.
MRP6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCC6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCC6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCC6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCC6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.