MRP5 CRISPR/Cas9 KO Plasmid (h): sc-402443
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRP5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP5 Antibody (E-10): sc-376965
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP5 CRISPR/Cas9 KO Plasmid (h) | sc-402443 | 20 µg | $397.00 |
Overview
ABCC5 encodes the human ATP-binding cassette transporter MRP5 (ABCC5), a membrane efflux pump that uses ATP hydrolysis to export a range of organic anions, including cyclic nucleotides such as cGMP, and other metabolites that influence intracellular signaling. By regulating cytosolic nucleotide pools and xenobiotic handling, MRP5 interfaces with processes linked to membrane transport, redox balance, and cellular stress responses. Altered ABCC5/MRP5 activity has been associated with changes in drug disposition phenotypes and with disease-relevant programs involving proliferation and survival in multiple tissue contexts. As a result, ABCC5 is frequently studied in pathways connecting transport-mediated homeostasis to cell state regulation.
MRP5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ABCC5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ABCC5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ABCC5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRP5 protein expression.
This CRISPR knockout system enables efficient generation of ABCC5-deficient cell models for investigation of MRP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ABCC5 exon(s) critical for MRP5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ABCC5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRP5 CRISPR/Cas9 KO Plasmid (h) and MRP5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ABCC5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRP5 HDR Plasmid (h) and MRP5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ABCC5 homology arms to support homology-directed repair at defined ABCC5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.