MRP3 Lentiviral Activation Particles (h): sc-401508-LAC

ABCC3 encodes the ATP-binding cassette transporter MRP3 (ABCC3), a basolateral efflux pump that exports a broad spectrum of organic anions including glucuronide, sulfate, and glutathione conjugates. MRP3 supports phase II detoxification by coupling conjugate formation to cellular export, influencing hepatic and intestinal xenobiotic handling, bile acid homeostasis, and inflammatory stress responses. Its activity intersects with drug disposition and transporter-regulated barrier functions, making ABCC3 a relevant node in pharmacology-oriented pathway studies and in models of hepatobiliary dysfunction, cholestasis-associated signaling, and transporter-mediated chemoresistance phenotypes. Dysregulated ABCC3 expression has been reported across several disease contexts where altered conjugated metabolite flux and redox balance contribute to cellular adaptation.

MRP3 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient ABCC3 upregulation across a broader range of human cell types.

MRP3 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the ABCC3 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous MRP3 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native ABCC3 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.