MRP2 CRISPR/Cas9 KO Plasmid (m): sc-419711
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP2 Antibody (M2III-5): sc-59611
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP2 CRISPR/Cas9 KO Plasmid (m) | sc-419711 | 20 µg | $397.00 |
Overview
Abcc2 encodes the ATP-binding cassette transporter MRP2 (ABCC2), a plasma membrane efflux pump that exports glutathione, glucuronide, and sulfate conjugates of endogenous metabolites and xenobiotics. In mouse, MRP2 supports cellular detoxification and redox homeostasis by coupling ATP hydrolysis to the transport of organic anions, including bilirubin conjugates and other phase II metabolites. Its activity intersects with hepatic and renal clearance pathways and contributes to barrier functions in polarized epithelia through coordinated trafficking and canalicular/apical localization. Altered ABCC2/MRP2 function is commonly studied in the context of cholestatic phenotypes, hyperbilirubinemia-related biology, and multidrug resistance mechanisms in model systems.
MRP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Abcc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Abcc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Abcc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRP2 protein expression.
This CRISPR knockout system enables efficient generation of Abcc2-deficient cell models for investigation of MRP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Abcc2 exon(s) critical for MRP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Abcc2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRP2 CRISPR/Cas9 KO Plasmid (m) and MRP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Abcc2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRP2 HDR Plasmid (m) and MRP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Abcc2 homology arms to support homology-directed repair at defined Abcc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.