MRP-S6 Double Nickase Plasmid (h): sc-413064-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP-S6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MRP-S6 Double Nickase Plasmid (h) and MRP-S6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MRPS6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP-S6 Antibody (E-8): sc-390597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP-S6 Double Nickase Plasmid (h) | sc-413064-NIC | 20 µg | $410.00 |
MRPS6 encodes the human mitochondrial ribosomal protein S6 (MRP-S6), a component of the 28S small subunit of the mitoribosome that supports translation of mitochondrially encoded oxidative phosphorylation subunits. By enabling efficient synthesis of respiratory chain proteins, MRP-S6 contributes to mitochondrial proteostasis, electron transport chain function, and cellular energy metabolism. Disruption of mitochondrial ribosome components can impair OXPHOS assembly and promote compensatory stress responses linked to altered redox balance and bioenergetic remodeling. MRPS6 is therefore relevant to studies of mitochondrial dysfunction mechanisms observed across diverse metabolic and neurodegeneration-associated phenotypes.
MRP-S6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MRPS6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MRPS6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MRPS6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MRPS6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.