MRP-S27 Antibody (A-10): sc-390396

MRP-S27 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects MRP-S27 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MRP-S27 (A-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRP-S27, also known as mitochondrial ribosomal protein S27 or S27mt, is a crucial component of the mitochondrial ribosome′s small subunit (28S), consisting of 414 amino acids and characterized by the presence of two pentatricopeptide repeats. MRP-S27 plays a vital role in mitochondrial protein synthesis, which is essential for proper functioning of mitochondria, the cell′s powerhouses. MRP-S27 is predominantly localized in mitochondria and is highly expressed in tissues with significant energy demands, such as skeletal muscle and heart. The gene encoding MRP-S27 is located on human chromosome 5, which spans 181 million base pairs and comprises nearly 6% of the human genome, with notable associations to various genetic disorders. Deletions on the p arm of chromosome 5 can lead to Cri du chat syndrome, while alterations on the q arm are frequently observed in therapy-related acute myelogenous leukemias and myelodysplastic syndromes. MRP-S27′s interaction with other mitochondrial ribosomal proteins underscores its importance in maintaining mitochondrial function and cellular energy metabolism.

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MRP-S27 Antibody (A-10) References:

1. The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present.  |  Cavdar Koc, E., et al. 2001. J Biol Chem. 276: 19363-74. PMID: 11279123
2. Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.  |  Koc, EC., et al. 2001. Protein Sci. 10: 471-81. PMID: 11344316
3. Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.  |  Zhang, Z. and Gerstein, M. 2003. Genomics. 81: 468-80. PMID: 12706105
4. Haematological & molecular profile of acute myelogenous leukaemia in India.  |  Sazawal, S., et al. 2009. Indian J Med Res. 129: 256-61. PMID: 19491417
5. Two further cases of myelodysplastic syndrome and acute myeloid leukemia with der(5;19)(p10;q10): association with abnormalities involving chromosomes 12 and 21.  |  Yamamoto, K., et al. 2010. Leuk Res. 34: e38-41. PMID: 19781776
6. Large distal 5p deletion with hemifacial microsomia and absence of cri-du-chat syndrome.  |  Wang, JC. and Khan, A. 2010. Clin Dysmorphol. 19: 38-39. PMID: 19923986
7. Enhanced expression of tubulin-specific chaperone protein A, mitochondrial ribosomal protein S27, and the DNA excision repair protein XPACCH in the song system of juvenile male zebra finches.  |  Qi, LM., et al. 2012. Dev Neurobiol. 72: 199-207. PMID: 21780305
8. MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.  |  Davies, SM., et al. 2012. FEBS Lett. 586: 3555-61. PMID: 22841715
9. Inter-Species Host Gene Expression Differences in Response to Human and Avian Influenza A Virus Strains.  |  Taye, B., et al. 2017. Int J Mol Sci. 18: PMID: 29104227
10. Expression analysis of mammalian mitochondrial ribosomal protein genes.  |  Cheong, A., et al. 2020. Gene Expr Patterns. 38: 119147. PMID: 32987154
11. Dysregulated Ribosome Biogenesis Is a Targetable Vulnerability in Triple-Negative Breast Cancer: MRPS27 as a Key Mediator of the Stemness-inhibitory Effect of Lovastatin.  |  Zheng, C., et al. 2024. Int J Biol Sci. 20: 2130-2148. PMID: 38617541
12. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.  |  Nagase, T., et al. 1996. DNA Res. 3: 321-9, 341-54. PMID: 9039502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRP-S27 Antibody (A-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MRP-S27 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRP-S27 Antibody (A-10) Specifications

Alternate Product Name: MRP-S27

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-55

Epitope Target: MRP-S27

Epitope Species: human

Product Reactivity/Detection: MRP-S27

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MRP-S27 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin