MRP-S18B CRISPR/Cas9 KO Plasmid (h): sc-412012
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP-S18B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRP-S18B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP-S18B CRISPR/Cas9 KO Plasmid (h) | sc-412012 | 20 µg | $397.00 |
Overview
MRPS18B encodes mitochondrial ribosomal protein S18B (MRP-S18B), a component of the 28S small subunit of the mammalian mitoribosome required for translation of mitochondrially encoded oxidative phosphorylation (OXPHOS) proteins. By supporting synthesis of key electron transport chain subunits, MRP-S18B contributes to mitochondrial respiratory capacity, ATP production, and redox homeostasis, linking it to pathways controlling mitochondrial proteostasis and integrated stress signaling. Altered mitochondrial translation and OXPHOS imbalance are recurrent features in disorders involving energy-demanding tissues and are frequently observed in cancer and neurodegeneration-associated mitochondrial dysfunction phenotypes. MRPS18B is therefore studied in contexts such as mitochondrial biogenesis, metabolic reprogramming, and nucleo-mitochondrial coordination.
MRP-S18B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MRPS18B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MRPS18B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MRPS18B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRP-S18B protein expression.
This CRISPR knockout system enables efficient generation of MRPS18B-deficient cell models for investigation of MRP-S18B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MRPS18B exon(s) critical for MRP-S18B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MRPS18B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRP-S18B CRISPR/Cas9 KO Plasmid (h) and MRP-S18B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MRPS18B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRP-S18B HDR Plasmid (h) and MRP-S18B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MRPS18B homology arms to support homology-directed repair at defined MRPS18B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.