MRP-L3 Antibody (B-3): sc-514217
- MRP-L3 Antibody (B-3) is a mouse monoclonal IgG2a κ MRP-L3 antibody provided at 200 µg/ml
- raised against amino acids 47-155 mapping near the N-terminus of MRP-L3 of human origin
- MRP-L3 Antibody (B-3) is recommended for detection of MRP-L3 of human origin by WB, IP, IF and ELISA
- Anti-MRP-L3 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MRP-L3 Antibody (B-3) for WB applications. These reagents are now offered in bundles with MRP-L3 Antibody (B-3) (see ordering information below).
MRP-L3 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects MRP-L3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MRP-L3 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRP-L3, also known as mitochondrial 39S ribosomal protein L3 or L3mt, is a crucial component of the mitochondrial ribosome, which is essential for protein synthesis within the mitochondrion. This 348 amino acid protein belongs to the ribosomal protein L3P family and is localized to the mitochondria, specifically within the 39S subunit of the mitoribosomes. The unique structure of MRP-L3, along with its interaction with other mitochondrial ribosomal proteins, plays a vital role in the assembly and function of the mitoribosome, which is critical for the translation of mitochondrial mRNA into proteins necessary for cellular respiration and energy production. The distinct composition of mitochondrial ribosomes, which consists of a 4:1 ratio of protein to RNA, highlights the importance of MRP-L3 in maintaining mitochondrial function and overall cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MRP-L3 Antibody (B-3) References:
- Mammalian mitochondrial ribosomal proteins (2). Amino acid sequencing, characterization, and identification of corresponding gene sequences. | O'Brien, TW., et al. 1999. J Biol Chem. 274: 36043-51. PMID: 10593885
- Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. | Simpson, JC., et al. 2000. EMBO Rep. 1: 287-92. PMID: 11256614
- The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. | Kenmochi, N., et al. 2001. Genomics. 77: 65-70. PMID: 11543634
- Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome. | Zhang, Z. and Gerstein, M. 2003. Genomics. 81: 468-80. PMID: 12706105
- Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells. | Ou, JH., et al. 1987. Nucleic Acids Res. 15: 8919-34. PMID: 2891103
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP-L3 Antibody (B-3) | sc-514217 | 200 µg/ml | $322.00 | |||
MRP-L3 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-526351 | 200 µg Ab; 10 µg BP | $361.00 | |||
MRP-L3 Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-546600 | 200 µg Ab; 10 µg BP | $361.00 | |||
MRP-L3 Antibody (B-3) AC | sc-514217 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MRP-L3 Antibody (B-3) HRP | sc-514217 HRP | 200 µg/ml | $322.00 | |||
MRP-L3 Antibody (B-3) FITC | sc-514217 FITC | 200 µg/ml | $336.00 | |||
MRP-L3 Antibody (B-3) PE | sc-514217 PE | 200 µg/ml | $349.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 488 | sc-514217 AF488 | 200 µg/ml | $364.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 546 | sc-514217 AF546 | 200 µg/ml | $364.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 594 | sc-514217 AF594 | 200 µg/ml | $364.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 647 | sc-514217 AF647 | 200 µg/ml | $364.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 680 | sc-514217 AF680 | 200 µg/ml | $364.00 | |||
MRP-L3 Antibody (B-3) Alexa Fluor® 790 | sc-514217 AF790 | 200 µg/ml | $364.00 |