MRP-L10 Antibody (E-9): sc-377196

MRP-L10 Antibody (E-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects MRP-L10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MRP-L10 (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRP-L10 protein, also known as 39S ribosomal protein L10, mitochondrial or MRPL8, is a crucial component of the mitochondrial ribosome, consisting of 261 amino acids. MRP-L10 plays a vital role in mitochondrial protein synthesis, which is essential for oxidative phosphorylation, a process that generates ATP, the energy currency of the cell. MRP-L10 is one of the 70 proteins that make up the mitochondrial ribosome, all encoded by the nuclear genome, highlighting the intricate relationship between nuclear and mitochondrial functions. MRP-L10 undergoes deacetylation in a NAD+-dependent manner by the enzyme SIRT3, a modification significant for the regulation of mitochondrial protein synthesis and cellular metabolism. MRP-L10 gene is located on human chromosome 17, a region rich in genetic information, comprising over 2.5% of the human genome and associated with key tumor suppressor genes such as p53 and BRCA1, underscoring potential implications in cancer biology and therapeutic research.

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MRP-L10 Antibody (E-9) References:

1. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.  |  Kenmochi, N., et al. 2001. Genomics. 77: 65-70. PMID: 11543634
2. The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present.  |  Koc, EC., et al. 2001. J Biol Chem. 276: 43958-69. PMID: 11551941
3. Molecular hierarchy in neurons differentiated from mouse ES cells containing a single human chromosome 21.  |  Wang, CC., et al. 2004. Biochem Biophys Res Commun. 314: 335-50. PMID: 14733910
4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).  |  Gerhard, DS., et al. 2004. Genome Res. 14: 2121-7. PMID: 15489334
5. DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.  |  Zody, MC., et al. 2006. Nature. 440: 1045-9. PMID: 16625196
6. NAD+-dependent deacetylase SIRT3 regulates mitochondrial protein synthesis by deacetylation of the ribosomal protein MRPL10.  |  Yang, Y., et al. 2010. J Biol Chem. 285: 7417-29. PMID: 20042612

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRP-L10 Antibody (E-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MRP-L10 Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRP-L10 Antibody (E-9) Specifications

Alternate Product Name: MRP-L10

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 167-195

Epitope Target: MRP-L10

Epitope Species: human

Product Reactivity/Detection: MRP-L10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MRP-L10 Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin