Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MPST Double Nickase Plasmid (h): sc-401643-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPST Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MPST Double Nickase Plasmid (h) and MPST Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MPST. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MPST Antibody (H-11): sc-376168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPST Double Nickase Plasmid (h) | sc-401643-NIC | 20 µg | $410.00 |
MPST (3-mercaptopyruvate sulfurtransferase) is a mitochondrial and cytosolic sulfurtransferase that catalyzes persulfide transfer reactions, contributing to endogenous hydrogen sulfide and polysulfide production from cysteine-derived substrates. Through regulation of cellular redox homeostasis and protein persulfidation, MPST influences oxidative stress responses, mitochondrial bioenergetics, and sulfur amino acid metabolism. Altered MPST activity has been linked to dysregulated reactive oxygen species handling and metabolic remodeling, making it relevant to studies of neurodegeneration, cardiovascular stress responses, and inflammation-associated signaling. MPST function is also examined in the context of ferroptosis sensitivity and thiol-dependent detoxification pathways, where sulfur transfer reactions can modulate cellular vulnerability to lipid peroxidation.
MPST Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MPST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MPST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MPST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MPST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.