mPR delta / gamma Antibody (B-8) recommended for detection of mPRδ and mPRγ of mouse, rat and human origin by WB, IP, IF and ELISA

mPRδ/γ Antibody (B-8): sc-514273

mPRδ/γ Antibody (B-8) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-mPRδ/γ Antibody (B-8) is a mouse monoclonal IgG1 (kappa light chain) mPRδ/γ antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 234-257 near the C-terminus of mPRγ of human origin
    • Anti-mPR delta / gamma Antibody (B-8) is recommended for detection of mPRδ and mPRγ of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-mPR delta / gamma Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514273 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of mPRδ/γ (B-8): sc-514273.
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For western blotting with this antibody, would it be better to block the membrane with milk and BSA or just milk prior to adding the primary antibody?

Asked by: sp15
Thank you for your question. We recommend blocking and diluting in 5% milk with TBS-T. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-08-28

What application is the blocking peptide sc-514273 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Not good for IF in rat brain tissue 1:50 dilution and the negative control showed no difference
Date published: 2020-01-16
Rated 5 out of 5 by from Great Western blot data of mPR / expression Great Western blot data of mPR / expression in HUV-EC-C and WI-38 whole cell lysates. -SCBT QC
Date published: 2013-05-28
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