MPP3 CRISPR/Cas9 KO Plasmid (m): sc-420007
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPP3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MPP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MPP3 Antibody (D-8): sc-377522
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPP3 CRISPR/Cas9 KO Plasmid (m) | sc-420007 | 20 µg | $397.00 |
Overview
Mpp3 encodes membrane palmitoylated protein 3 (MPP3), a member of the MAGUK family of scaffold proteins that organize multiprotein complexes at the plasma membrane. MPP3 contributes to cell polarity and junctional architecture by linking transmembrane partners to cytoskeletal and signaling components through its PDZ, SH3, and guanylate kinase-like domains. In mouse tissues, MPP3 is implicated in epithelial organization and vesicular/membrane trafficking processes that influence differentiation and barrier functions. Dysregulation of MAGUK scaffolds is broadly associated with altered adhesion-dependent signaling and tissue disorganization, making Mpp3 a useful target for studying mechanisms relevant to development and disease-associated changes in cellular architecture.
MPP3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mpp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mpp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mpp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MPP3 protein expression.
This CRISPR knockout system enables efficient generation of Mpp3-deficient cell models for investigation of MPP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mpp3 exon(s) critical for MPP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mpp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MPP3 CRISPR/Cas9 KO Plasmid (m) and MPP3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mpp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MPP3 HDR Plasmid (m) and MPP3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mpp3 homology arms to support homology-directed repair at defined Mpp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.