MPP10 Antibody (TJ-1): sc-81847

MPP10 Antibody (TJ-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MPP10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MPP10 antibody (TJ-1) is available as the non-conjugated format. MPP10, also known as M-phase phosphoprotein 10 or MPHOSPH10, is a 681 amino acid protein that plays a crucial role in ribosomal RNA processing, specifically during the M phase of the cell cycle. MPP10 is localized to the fibrillar region of the nucleolus, where phosphorylation occurs to become a key component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. This complex is essential for the early cleavages during pre-18S ribosomal RNA processing, highlighting MPP10′s importance in the synthesis of ribosomes, which are vital for protein production in all living cells. MPP10 forms a heterotrimeric complex with IMP-3 and IMP-4, which may facilitate interaction with other nucleolar components, thereby influencing ribosome biogenesis. The gene encoding MPP10 is located on human chromosome 2, a chromosome that contains over 500 genes and is associated with various genetic disorders, including Phelan-McDermid syndrome and Neurofibromatosis type 2. MPP10′s ability to interact with specific proteins and involvement in critical cellular processes make MPP10 a significant target for research in cell biology and disease mechanisms.

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MPP10 Antibody (TJ-1) References:

1. Functional proteomic analysis of human nucleolus.  |  Scherl, A., et al. 2002. Mol Biol Cell. 13: 4100-9. PMID: 12429849
2. The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60-80S ribonucleoprotein complexes.  |  Granneman, S., et al. 2003. Nucleic Acids Res. 31: 1877-87. PMID: 12655004
3. The small-subunit processome is a ribosome assembly intermediate.  |  Bernstein, KA., et al. 2004. Eukaryot Cell. 3: 1619-26. PMID: 15590835
4. Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes.  |  Segerstolpe, A., et al. 2008. Nucleic Acids Res. 36: 4364-80. PMID: 18586827
5. A novel small-subunit processome assembly intermediate that contains the U3 snoRNP, nucleolin, RRP5, and DBP4.  |  Turner, AJ., et al. 2009. Mol Cell Biol. 29: 3007-17. PMID: 19332556
6. MPP1 interacts with DOPC/SM/Cholesterol in an artificial membrane system using Langmuir-Blodgett monolayer.  |  Elderdfi, M., et al. 2017. Gen Physiol Biophys. 36: 443-454. PMID: 28653654
7. MPP1-based mechanism of resting state raft organization in the plasma membrane. Is it a general or specialized mechanism in erythroid cells?  |  Trybus, M., et al. 2019. Folia Histochem Cytobiol. 57: 43-55. PMID: 31099889
8. Molecular characterization of direct interactions between MPP1 and flotillins.  |  Biernatowska, A., et al. 2021. Sci Rep. 11: 14751. PMID: 34285255
9. Identification of membrane palmitoylated protein 1 (MPP1) as a heart-failure-promoting protein triggered by cardiovascular risk factors and aging.  |  Alla, JA., et al. 2023. Biochem Pharmacol. 217: 115789. PMID: 37683843
10. Identification of novel M phase phosphoproteins by expression cloning.  |  Matsumoto-Taniura, N., et al. 1996. Mol Biol Cell. 7: 1455-69. PMID: 8885239
11. M phase phosphoprotein 10 is a human U3 small nucleolar ribonucleoprotein component.  |  Westendorf, JM., et al. 1998. Mol Biol Cell. 9: 437-49. PMID: 9450966
12. Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors.  |  Baserga, SJ., et al. 1997. Nucleic Acids Symp Ser. 64-7. PMID: 9478208

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MPP10 Antibody (TJ-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MPP10 Antibody (TJ-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MPP10 Antibody (TJ-1) Specifications

Alternate Product Name: MPP10

Product Clone Name: TJ-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 348-458

Epitope Target: MPP10

Epitope Species: human

Product Reactivity/Detection: MPP10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MPP10 Antibody (TJ-1): N/A